Concomitant Administration of Catharanthus roseus Extract Improves Efficacy and Safety by Nullifying Diarrhea-Related Toxicity of Acarbose and Metformin in Wistar Rats

Sylvester C. Ohadoma1*, Louis U. Amazu2, Christian E. Okolo2, Leo C. Chukwu3, Henry U. Michael4
1Department of Pharmacology, College of Medical Sciences, University of Calabar, Nigeria
2Department of Pharmacology, College of Medicine, Imo State University, Owerri, Nigeria
3Department of Pharmacology, College of Medicine; Chukwuemeka Odumegwu Ojukwu University, Amaku Awka Campus Nigeria
4Discipline of Pharmaceutical Sciences, School of Health Sciences, University of Kwazulu-Natal,  Durban, South Africa

Corresponding Author: chodraf@yahoo.com; Tel: +2348035081946
Recieved Date: September 03, 2020; Accepted Date: October 03, 2020; Published Date: 03 October 2020
Citation: Ohadoma SC, Amazu LU, Okolo CE, Chukwu LC, Michael HU. Concomitant Administration of Catharanthus roseus Extract Improves Efficacy and Safety by Nullifying Diarrhea-Related Toxicity of Acarbose and Metformin in Wistar Rats. Trop J Nat Prod Res. 2020; 4(9):649-652.  https://doi.org/10.26538/tjnpr/v4i9.25
Copyright: © 2020 Ohadoma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The study conceptualized the sites and mechanisms of action as well as explored the interacting benefits of Catharanthus roseus (C. roseus) against the diarrhea-like toxicity associated with the hypoglycemic activity of acarbose and metformin using experimental rats. The diabetes was induced with alloxan. Diarrhoea was  induced with castor oil (1 ml per rat) after 30 min of determining blood glucose levels of the different groups including additional Group VIII which received loperamide (3 mg/kg) serving as positive control. The sugar level was determined using a glucometer; while inhibition percent of fecal count and fecal weight were employed to determine antidiarrheal activity. All medicaments showed significant lowering of blood glucose levels when compared with control alone (P < 0.05) having the combination of extract-metformin-acabose extremely significant (P < 0.01), followed by extract-metformin. Also, significant antidiarrheal activity was shown in all groups treated with C. roseus, with highest percentage inhibition in fecal count 86.72% and fecal weight 89.78% exhibited by extract-metformin-acarbose combination compared to loperamide (86.74 and 81.17% respectively). The extract of C. roseus enhances hypoglycemic efficacy and safety by significantly nullifying diarrheal-like adverse effects of acarbose and metformin.

Keywords: Catharanthus roseus, Acarbose, Metformin, diarrhea-related toxicity, concomitant administration. 
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