Phytochemical, Antioxidant and Anti-Inflammatory Characterization of Leaves and Bark of Mimusops coriacea (A.DC) Miq from Ecuador

Katherine E. Bustamante-Pesantes1, Yamilet I. Gutiérrez-Gaitén2, Oswaldo G. Pesantes Domínguez2; Migdalia Miranda-Martínez3*
1Guayaquil University. Faculty of Chemical Sciences. Guayaquil. Ecuador 
2Department of Pharmacy, Institute of Pharmacy and Food, University of Havana. Havana Cuba
3Superior Polytechnic School of the Coast. ESPOL. Department of Chemical and Environmental Sciences. Faculty of Natural Sciences and Mathematics Guayaquil. Ecuador

Corresponding Author: mgmiran@espol.edu.ec; Tel: +593 0988408956
Recieved Date: August 26, 2020; Accepted Date: October 03, 2020; Published Date: 03 October 2020
Citation: Bustamante-Pesantes KE, Gutiérrez-Gaitén YI, Pesantes Domínguez OG, Miranda-Martínez M.Phytochemical, Antioxidant and Anti-Inflammatory Characterization of Leaves and Bark of Mimusops Coriacea (A.DC) Miq from Ecuador. Trop J Nat Prod Res. 2020; 4(9):578-585.  https://doi.org/10.26538/tjnpr/v4i9.14
Copyright: © 2020 Bustamante-Pesantes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Mimusops coriacea (A.DC) belonging to the family Sapotacea is a tree that grows widely in Ecuador. It is traditionally used as an analgesic and anti-inflammatory medicine. There is no phytochemical information on the leaves and bark of the plant, or pharmacological studies that justifies its traditional use. The objective of this study was to determine the chemical composition, antioxidant and anti-inflammatory activity of hydroalcoholic extracts of leaves and bark of M. coriacea. Extracts were obtained by maceration from leaves and bark, using 80% hydroalcoholic mixture as solvent. Total phenols were quantified by Folin-Ciocalteu, total flavonoids by the colorimetric method of aluminum chloride and an analysis was performed by LC-MS. The antioxidant capacity by FRAP, DPPH and ABTS assays and anti-inflammatory activity using carrageenan induced paw edema model in rats were evaluated. The results revealed a significant difference in the content of phenols and flavonoids, being higher for the leaf extract. Two glycosylated flavonoids (myricetin-3-O-?-L-rhamnoside and myricetin-3-O-glucoside) and two triterpenic saponins derived from protobasic acid were identified by LC-MS. The two extracts showed a high ferro-reducing capacity and antiradical activity, with the leaf extract being the most active (IC50 4.58 ?g/mL, DPPH and 197.00 ?g/mL, ABTS). Both the extract from the leaves and the bark showed an anti-inflammatory effect, somewhat higher than the extract from the leaves.  The results provide the first findings on the chemical and biological study of the leaves and bark of M. coriacea that grow in Ecuador, which justifies the traditional use of the species as anti-inflammatory. 

Keywords: Antioxidant activity, Anti-inflammatory activity, Barks, Leaves, Mimusops coriacea, Phytochemical.
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