Antibacterial Activity of Dipterocarpus alatus Twig Extract against Methicillin-Resistant Staphylococcus aureus (MRSA)

Waranya Chatuphonprasert1,2, Nitima Tatiya-aphiradee2, Sutthiwan Thammawat1, Yollada Sriset2, Ploenthip Puthongking4, Bunleu Sungthong3, Nadta Sukkasem2, Nathaphon Kuncharoenwirat2, Kanokwan Jarukamjorn2,4*
1Faculty of Medicine, Mahasarakham University, Muang, Maha Sarakham 44000 Thailand
2Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Khon Kaen University, Khon Kaen 40002 Thailand
3Pharmaceutical Chemistry and Natural Products Research Unit, Faculty of Pharmacy, Mahasarakham University, Kantharawichai, Maha Sarakham, 44150 Thailand
4Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand


Corresponding Author: kanok_ja@kku.ac.th; Tel: +66-043-202379
Recieved Date: August 21, 2020; Accepted Date: October 02, 2020; Published Date: 03 October 2020
Citation: Chatuphonprasert W, Tatiya-aphiradee N, Thammawat S, Sriset, Y, Puthongking, P, Sungthong, B, Sukkasem, N, Kuncharoenwirat, N, Jarukamjorn, K. Antibacterial Activity of Dipterocarpus alatus Twig Extract against Methicillin-Resistant Staphylococcus aureus (MRSA). Trop J Nat Prod Res. 2020; 4(9):571-577.  https://doi.org/10.26538/tjnpr/v4i9.13
Copyright: © 2020 Chatuphonprasert et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Dipterocarpus alatus has been used for the treatment of external wounds and skin diseases in traditional medicine. Staphylococcus aureus is a major pathogen for human superficial skin infections. This study determined the antibacterial effects of D. alatus twig crude extracts against methicillin-sensitive/resistant S. aureus (MSSA and MRSA) in vitro and in vivo. Several organic solvents were used to extract D. alatus twig. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of the D. alatus extracts for MSSA and MRSA were investigated using broth micro-dilution assays. The most potent extract was studied in a mouse superficial wound model with MRSA infection. The ethyl acetate-methanol extract of D. alatus,  MeOH(EtOAc), showed the lowest MIC and MBC values (0.390 and 0.781 mg/mL, respectively) against both MSSA and MRSA compared to the other extracts. MRSA-infected mouse superficial wounds showed increased transepidermal water loss (TEWL), mast cell accumulation, and MRSA colony numbers. Treatment with MeOH(EtOAc) extract (20 mg/mL), or MeOH(EtOAc) emulgel (20 - 40 mg/mL), 100 µL applied daily for 10 days, reduced TEWL values, mast cell accumulation, and MRSA colony numbers in wounds compared to the non-treated group. The wounds of the MeOH(EtOAc) extract and MeOH(EtOAc) emulgel treatment groups were healed on day 8 while the wounds of the dipterocarpol and tetracycline treatment groups were not. Therefore, MeOH(EtOAc) extract or MeOH(EtOAc) emulgel are good candidates for a topical product to treat ulcerated wounds with MRSA infection.

Keywords: Dipterocarpol, Resin tree, MRSA, MSSA, Antibacterial activity, Wound healing.
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