Tannin Screening, Phenolic Compounds Analysis, and Antiproliferative Activity of Areca Nut Extract by Decreasing Ki-67 Protein in Oral Squamous Carcinoma Cell Lines

Liza M. Sari1*, Dewi Wulandari2, Arleni Bustami3, Gus P. Subita4, Elza I. Auerkari5
1Department of Oral Medicine, Faculty of Dentistry, University of Syiah Kuala, Banda Aceh, Indonesia
2Department of Clinical Pathology, Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia 
3Integrated Cytogenetic and Cell Culture Laboratory, Cipto Mangunkusumo Hospital, University of Indonesia, Jakarta, Indonesia 
4Department of Oral Medicine, Faculty of Dentistry, University of Indonesia, Jakarta, Indonesia
5Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta, Indonesia

Corresponding Author: lizameutiasari@unsyiah.ac.id; Tel: +62-87886497414
Recieved Date: August 12, 2020; Accepted Date: September 30, 2020; Published Date: 03 October 2020
Citation: Sari LM, Wulandari D, Bustami A, Subita GP, Auerkari EI. Tannin Screening, Phenolic Compounds Analysis, and Antiproliferative Activity of Areca Nut Extract by Decreasing Ki-67 Protein in Oral Squamous Carcinoma Cell Lines. Trop J Nat Prod Res. 2020; 4(9):563-570.  https://doi.org/10.26538/tjnpr/v4i9.12
Copyright: © 2020 Sari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The target of cancer therapy based on chemoradiotherapy or herbal medicine is to inhibit the cancer cell proliferation.  Due to the current interest in the potential activity of areca nut extract, we investigated its ability to affect cell-cycle arrest and Ki-67 activity in Oral Squamous Carcinoma (OSC) cell lines. The tannin content was estimated by titrimetric analysis. The phenolic composition was analyzed using Liquid Chromatography-Mass Spectrometry (LC-MS). Flow cytometry was performed for analyzing cell-cycle arrest and Ki-67 protein as an indicator of the proliferative activity in HSC-2 and HSC-3 cell lines. The areca nut extract showed 0.007% tannin, catechin, and quercetin. The cell-cycle arrest occurred in HSC-3 cells. The reduction of Ki-67 activities occurred in both cells after 24 hours. The areca nut extract has antiproliferative activity in OSC cell lines. 

Keywords: Tannin, Catechin, Quercetin, Oral squamous carcinoma, Cell-cycle.
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