Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis

Rajamanickam Buddhan1, Shanmugam Manoharan1, Chakkaravarthy Elanchezhiyan2,  Radhakrishnan Muralinaidu3,  Mohan Karthik1
1Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, India
2Department of Zoology, Annamalai University, Annamalainagar, Tamil Nadu, India
3Department of Oral Pathology, Rajah Muthiah Dental College, Annamalai University, Annamalainagar, Tamil Nadu, India


Corresponding Author: sakshiman@rediffmail.com; Tel: +91 94425 48117
Recieved Date: August 06, 2020; Accepted Date: September 25, 2020; Published Date: 03 October 2020
Citation: Buddhan R, Manoharan S, Elanchezhiyan C,  Muralinaidu R, Karthik M. Myrtenal Modulates the Immunoexpression of Cell Proliferative, Angiogenic and Invasive Markers in DMBA-Induced Hamster Oral Carcinogenesis. Trop J Nat Prod Res. 2020; 4(9):550-557.   https://doi.org/10.26538/tjnpr/v4i9.10
Copyright: © 2020 Buddhan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Abnormal cell proliferation, invasion, metastasis and angiogenesis are the most prominent features of malignant tumors. The present study evaluated the modulating effect of myrtenal on the immunoexpression pattern of cell proliferative (PCNA and cyclin D1), angiogenic (VEGF) and invasive (MMP-2 and MMP-9) markers in 7,12-dimethylbenz(a)anthracene (DMBA)- induced experimental oral carcinogenesis in golden Syrian hamsters using immunohistochemical assay. Topical application (painting) of 0.5% DMBA (six hamsters), a site specific carcinogen, three times a week for 14 weeks resulted in the formation of tumors in the buccal pouches of golden Syrian hamsters , which was confirmed by histopathological studies. Buccal mucosa excised from the hamsters treated with DMBA alone (tumor bearing hamsters) showed abnormal immunoexpression pattern of cell proliferative, angiogenic and invasive markers. Myrtenal administration (230 mg/kg b.w) orally to DMBA treated hamsters significantly downregulated the expression of the above said molecular markers in the chemopreventive phase and considerably decreased the expression pattern in the chemotherapeutic phase. The findings from this study, thus support the anti-cell proliferative, anti-angiogenic, and anti-invasive potential of myrtenal in DMBA-induced hamster buccal pouch carcinoma. 

Keywords: Cancer, DMBA, hamsters, cell proliferation, angiogenesis, invasion.
Back to Articles