Phytochemical Constituents of Securigera securidaca Seed Extract Using GS-MS and HPLC

Hammad K Aldalin1, Ghassab Al-Mazaideh2, Afaf H. Al-Nadaf3*, Fuad Al-Rimawi4, Akef T. Afaneh5, Ali Marashdeh5, Salih Abu-Lafi6, Fadi Alakhras7, Haya Ayyal-Salman8, Rasheed MAQ Jamhour9
1Department of Medical Support, Al-Balqa Applied University, Al-Karak University College, Al- Karak, Jordan
2Department of Chemistry and Chemical Technology, University of Tafila Technical University, Tafila, Jordan
3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mutah University, Alkarak- Jordan
4Chemistry Department, Faculty of Science and Technology, Al-Quds University, Jerusalem, Palestine 
5Department of Chemistry, Faculty of Science, Al-Balqa Applied University, Al-Salt, Jordan
6Faculty of Pharmacy, Al-Quds University, P.O. Box 20002, Jerusalem, Palestine
7Department of Chemistry, College of Science, Imam Abdulrahman Bin Faisal University, Dammam Saudi Arabia
8School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia
9Department of Chemistry and Chemical Technology, Faculty of Science, Tafila Technical University, Tafila –Jordan


Corresponding Author: a_nadaf@mutah.edu.jo; afaf_hn@yahoo.com; Tel: 00962 3 2386287 ext.6712
Recieved Date: August 02, 2020; Accepted Date: September 28, 2020; Published Date: 03 October 2020
Citation: Aldalin H K, Al-Mazaideh G, Al-Nadaf A H, Al-Rimawi F, Afaneh AT, Marashdeh A, Abu-Lafi S, Alakhras F, Ayyal-Salman H, Jamhour RMAQ. Phytochemical Constituents of Securigera securidaca Seed Extract Using GS-MS and HPLC. Trop J Nat Prod Res. 2020; 4(9):540-544.  https://doi.org/10.26538/tjnpr/v4i9.8
Copyright: © 2020 Aldalin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Securigera securidaca (L.) seeds are used in Jordanian folk medicine to treat several disorders like diabetes and hyperlipidemia. The aim of this study was to evaluate the chemical composition of S. securidaca methanol extract. The methanol extract of S. securidaca seeds obtained from local herbal shop in Jordan was investigated for its phytochemical composition using GC-MS and HPLC instruments.  Results showed that the methanol extract has twenty-seven natural compounds which made up 99.5% of the total composition. The HPLC-PDA results of S. securidaca showed many phytochemicals that are mainly composed of aromatics and oxygenated hydrocarbons. Dodecanedioic acid and its derivatives, as well as ?-Sitosterol are pharmacologically active components that have not been reported before in this plant. Three unidentified peaks originated in the GC-MS. Further detailed study is needed to investigate their identity. These results provide fine evidence for the ethno-pharmacological use of this plant to treat diabetes and hyperlipidemia. 

Keywords: Securigera securidaca, Phytochemical, Dodecanedioic acid, ?-Sitosterol.
Back to Articles