Toxicity Evaluation of Ethanol Extract of Lansium domesticum cv kokossan Seeds in Female Wistar Rats

Tri Mayanti1, Darwati Darwati1, Jamaludin Al Anshori1, Unang Supratman1,5, Madihah Madihah2, Ronny Lesmana3,5, Deden I. Dinata4, Shabarni Gaffar1*
1Department of Chemistry, Faculty of Mathematics and Natural Sciences  Universitas Padjadjaran, Sumedang, West Java,, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, West Java, Indonesia
3Departement of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
4Bandung School of Pharmacy, Bandung, Indonesia
5Central Laboratory, Universitas Padjadjaran, Sumedang, West Java, Indonesia

Corresponding Author: shabarni.gaffar@unpad.ac.id; Tel: +62-22-7794391
Recieved Date: July 16, 2020; Accepted Date: August 11, 2020; Published Date: 28 August 2020
Citation: Mayanti T, Darwati D, Al Anshori J, Supratman U, Madihah M, Lesmana R, Dinata DI, Gaffar S. Toxicity Evaluation of Ethanol Extract of Lansium domesticum cv kokossan Seeds in Female Wistar Rats. Trop J Nat Prod Res. 2020; 4(8):348-354.  https://doi.org/10.26538/tjnpr/v4i8.5
Copyright: © 2020 Mayanti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The seed extract of Lansium domesticum cv kokossan contains a new tetranotriterpenoid compound, kokosanolide A, that has the potential to be used as an anticancer agent. This study aims to evaluate the acute toxicity level of the ethanol extract of L. domesticum cv kokossan seed in female Wistar rats. The experiment was done based on OECD 425:2006 guidelines. The rats were divided into six groups, the amount of ethanol extract of L. domesticum cv kokossan seeds that were given to group I-IV were 5, 50, 500 and 5000 mg/kg BW, respectively. Group V was DMSO as vehicle control and group VI was doxorubicin as a positive control. The results showed a strong negative correlation between dose of the extract and the weight gain of tested animals (r = -0.93). The administration of the extract increased the relative weight of organs only at a high dose. Histopathological examination showed a sublethal manifestation of cell damage in the form of vacuolization of hydropic and fatty acid degeneration, particularly in hepatocytes of liver and renal tubules cells. The toxic signs, organ damage and liver enzyme biomarkers did not show significant difference compared to the control. The pseudo LD50 value estimated by Probit analysis was above 5000 mg/kg BW for ethanol extract, which categorized as relatively harmless. The findings showed non-toxic effects of the ethanol extracts of L. domesticum cv kokosan seeds on the female Wistar rat after acute administration. 

Keywords: Acute toxicity, Ethanol extract, Lansium domesticum seed, Rats.
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