Aqueous Extract of Phyllanthus amarus Schum & Thonn Leaves Attenuated the Alterations in Fluoxetine-Induced Anti-Oestrogenic Activity in Female Wistar Rats

Quadri O. Nurudeen* and Musa T. Yakubu
Phytomedicine, Toxicology and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, University of Ilorin, Ilorin, Nigeria.
Corresponding Author: quadriolaide@yahoo.com; Tel: +234-803-4273-045
Recieved Date: June 30, 2019; Accepted Date: July 25, 2020; Published Date: 27 July 2020
Citation: Nurudeen QO and Yakubu MT. Aqueous Extract of Phyllanthus amarus Schum & Thonn Leaves Attenuated the Alterations in Fluoxetine-Induced Anti-Oestrogenic Activity in Female Wistar Rats.  Trop J Nat Prod Res. 2020; 4(7):310-314.  https://doi.org/10.26538/tjnpr/v4i7.10
Copyright: © 2020 Nurudeen and Yakubu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Phyllanthus amarus leaves is widely acclaimed to be significant in the management of female sexual inadequacies. This study aimed to investigate the effects of aqueous extract of Phyllanthus amarus (Schum & Thonn) leaves (AEPAL) on the oestrogenic and uterine functioning indices of fluoxetine-treated female rats. Thirty healthy, sexually-responsive female rats (141.52±6.26g) were divided into six groups (A–F) comprising of 5 rats each. The animals in Group A (control group) were administered distilled water only, whereas those in Groups B,C,D,E, and F received orally 15 mg/kg body weight (b.w) of fluoxetine prepared daily in distilled water for 14 days for the induction of anti-oestrogenicity and subsequently received 0.5 mL of distilled water, 10 mg/kg b.w of a reference drug (Tadalafil) and 0.5 mL equivalent to 20, 40 and 80 mg/kg b.w of the extract respectively, orally, once daily (08:00-08:45h) for 7 days. The concentrations of total protein, total cholesterol, glycogen and oestrogen, along with the activities of alkaline phosphatase and acid phosphatase in the ovaries and uteri of the animals were significantly reduced (p<0.05) following the administration of fluoxetine. In contrast, these reductions were overturned by the AEPAL towards the control group. The extract at 80 mg/kg b.w ameliorated the reduced concentrations of these oestrogenic indices when compared with the non-sexually dysfunction control animals. The effect of Phyllanthus amarus leaves on endogenous estrogen activity and induction of gonadotrophin synthesis or secretion may further lend backing to the widespread use of Phyllanthus amarus leaves in handling some sterility/infertility problems in women.

Keywords: Oestrogenic activity, Phyllanthus amarus, Euphorbiaceae, Fluoxetine, Progesterone.
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