Phospholipase A2 from Naja nigricollis and Inhibitory Activity of Extracts from Commiphora africana (A. Rich.) Engl. (Burseraceae)

Abdulmumin Z. Abubakar*, Abdulrahman Adamu, Umar F. Shehu, Zainab Mohammed, Garba Ibrahim, Mujtaba S. Abubakar
Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria, Nigeria.
Corresponding Author: azabubakar01@gmail.com; azabubakar@abu.edu.ng; Tel: +2348065904149
Recieved Date: June 24, 2019; Accepted Date: July 22, 2020; Published Date: 27 July 2020
Citation: Abubakar AZ, Adamu A, Shehu UF, Mohammed Z, Ibrahim G, Abubakar MS. Phospholipase A2 from Naja nigricollis and Inhibitory Activity of Extracts from Commiphora africana (A. Rich.) Engl. (Burseraceae). Trop J Nat Prod Res. 2020; 4(7):286-291.  https://doi.org/10.26538/tjnpr/v4i7.6
Copyright: © 2020 Abubakar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Snakebite envenomation is a neglected global health problem responsible for substantial mortality, disability and psychological morbidity especially in rural tropical and subtropical regions of the world. Phospholipase A2 (PLA2s) enzymes constitute a major toxic venom component especially the venoms from elapids. Naja nigricollis is of the family Elapidae. Commiphora africana is a species of the family Burseraceae which is traditionally used in many parts of Africa for the treatment of number of ailments including snake envenomation, cancer and some inflammatory diseases. The present study was designed to determine the hydrolytic actions of PLA2 present in the N. nigricollis venom in an egg yolk suspension mixture and to access the PLA2 inhibitory effects of extracts of C. africana by acidimetric assay method. The snake venom PLA2 was found to release free fatty acids from the egg yolk suspension in a dose-dependent manner. The venom concentration of 0.1 mg/mL was found to liberate 13.3 µmol of free fatty acids with a decline of pH of 0.1 which may be attributable to the phospholipase A2 in the venom. Higher liberations of 133 µmol levels of free fatty acids was achieved with a decrease of pH of 1.0 at concentrations of 4.0 mg/mL of the venom. The PLA2 inhibitory activity observed from the extracts and fractions also showed concentration-dependent inhibition as depicted by the IC50 values. These results may provide some scientific basis for the usefulness of the plant in the traditional management of snakebite envenomation across Africa.

Keywords: Phospholipases A2, Commiphora africana, Naja nigricollis, inhibition.
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