Antibacterial Activity and Identification of Metabolites from the Semi-Purified Fraction of Chrysophyllum albidum Leaf (African Star Apple)

Ifeanyi Azih1, Magnus Ijezie1, Sylvester N. Ugariogu1, 2, Christogonus O. Akalezi1, 2
1Department of Chemistry, School of Physical Sciences, Federal University of Technology Owerri (FUTO), PMB 1526 Owerri, Nigeria.
2African Center of Excellence in Future Energies and electrochemical Systems,Federal University of Technology Owerri (FUTO), PMB 1526 Owerri, Nigeria

Corresponding Author: chrisakalezi@yahoo.com; Tel: +2348080587128
Recieved Date: May 27, 2019; Accepted Date: July 23, 2020; Published Date: 27 July 2020
Citation: Azih I, Ijezie M, Ugariogu SN, Akalezi CO. Antibacterial activity and identification of metabolites from the semi-purified fraction of Chrysophyllum albidum leaf (African star apple). Trop J Nat Prod Res. 2020; 4(7):262-269.  https://doi.org/10.26538/tjnpr/v4i7.2
Copyright: © 2020 Azih et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The medicinal uses of natural products especially medicinal plants have increased tremendously due to the multidrug resistance of some microorganisms and due to the availability and affordability of these plants. This study was aimed at the identification of metabolites from the fractions of Chrysophyllum albidium and their antibacterial activity. The phytochemical analysis and  antibacterial analysis were done using standard methods; Harborne and Agar-disc diffusion methods, respectively. The metabolite identification was done using infra-red (IR) and gas chromatography – mass spectrometry (GC-MS). The preliminary result of the antibacterbial screening showed that the neutral metabolite possessed the highest antibacterial activity and was then chosen for the IR and GC-MS study and purified using preparative thin layer chromatography. Various fractions were obtained from the chromatographic purification and in particular N3 fraction demonstrated the highest antibacterial activity against the test microbes with the zones of inhibition indicated against the test organism as follows: Streptococcus spp (18 mm), Staphylococcus aureus (20 mm), Salmonella typhi (25 mm), Escherichia coli (20 mm) and no inhibition zone against Enterobacter cloacae. The results confirm that the plant has some antibacterial activity. 

Keywords: Chrysophyllum albidium, Metabolites identification, Phytochemicals, Antibacterial.
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