Evaluation of the Anti-inflammatory and Antioxidant Activities of Selected Resin Exudates 

Dalia E. Ali1, Rehab S. Abdelrahman2,3, Rania A. El Gedaily4, Shahira M. Ezzat4,5, Meselhy R. Meselhy4,  Essam  Abdel-Sattar4*  
1Department of Pharmacognosy, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
3Department of Pharmacology and Toxicology, College of Pharmacy, Taibah University, Al-Madina Al-Munawwarah 30001, Saudi Arabia.
4Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, 11562 Cairo, Egypt.
5Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Science and Arts (MSA), 6th October, 12451 Egypt.

Corresponding Author: essam.abdelsattar@pharma.cu.edu.eg; Tel: +201065847211
Recieved Date: June 26, 2019; Accepted Date: July 25, 2020; Published Date: 27 July 2020
Citation: Ali DE, Abdelrahman RS, El Gedaily RA, Ezzat SM, Meselhy MR, Abdel-Sattar E. Evaluation of the Anti-inflammatory and Antioxidant Activities of Selected Resin Exudates. Trop J Nat Prod Res. 2020; 4(7):255-261.  https://doi.org/10.26538/tjnpr/v4i7.1
Copyright: © 2020 Ali et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Plant resins are reported to have high medicinal values due to their content of bioactive metabolites. Few reports were found in the last two decades concerning the chemistry and anti-inflammatory activity of the resins belonging to Eucalyptus and Araucaria genera. Therefore, the exudate resins of Eucalyptus maculata, Araucaria excelsa and Araucaria bidwillii were evaluated for their phenolic and flavonoid content, together with their possible anti-inflammatory potential via carrageenan-induced paw edema in rats at the doses of 100, 200, and 400 mg/kg. The methanol extract of E. maculata (MEME) kino resin was the richest in phenolics (572.82 ± 1.75 µg GAE/mg extract), and flavonoids (11.60 ± 0.01 µg QE/mg extract). The MEME (400 mg/kg) showed the most significant percentage inhibition of paw oedema (50.91%), compared to indomethacin (66.11%). The extract also decreased serum malondialdehyde (MDA) (42%), nuclear factor kappa B (NF-?B) (42.7%), tumor necrosis factor-alpha (TNF-?) (40.5%), cyclooxygenase-2 (COX-2) (42%) and nitric oxide (NO) (23%). In addition, it increased antioxidant enzymes; reduced glutathione (GSH) (2.6-fold) and superoxide dismutase (SOD) (1.3-fold), compared to control group. Moreover, it displayed a profound antioxidant property with IC50 of 6.83 ± 0.77 ?g/mL compared to trolox (IC50 21.18 ± 0.59 ?g/mL) using DPPH assay. The methanol extract of E. maculata resin was standardized by HPLC to contain 68.21 mg of 7-O-methyl aromadendrin (MA)/g extract. These findings suggest that E. maculata kino resin is a potent antioxidant with potential therapeutic efficacy for treating inflammation.

Keywords: Eucalyptus maculata, Araucaria excelsa, Araucaria bidwillii, Resin exudate, Inflammatory biomarkers, Antioxidant.
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