Antioxidant and Antibacterial Activity of Alkaloid Fractions of Tristemma hirtum P. Beauv

Basil N. Ita1* and Samuel I. Eduok2
1Department of Chemistry, University of Uyo, Nigeria.
2Department of Microbiology, University of Uyo, Nigeria.

Corresponding Author: basil_ita@yahoo.com; Tel: +2348023348014
Recieved Date: April 08, 2020; Accepted Date: April 25, 2020; Published Date: 30 April 2020
Citation: Ita BN and Eduok SI. Antioxidant and Antibacterial Activity of Alkaloid Fractions of Tristemma hirtum P. Beauv. Trop J Nat Prod Res. 2020; 4(4):179-184.  https://doi.org/10.26538/tjnpr/v4i4.10
Copyright: © 2020 Ita and Eduok . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Tristemma hirtum is a medicinal plant used in Nigerian traditional medicine to treat various diseases. In this study, the in vitro antioxidant and antibacterial activity of alkaloid fractions from organs of T. hirtum was evaluated. The antioxidant activity of the alkaloid fractions was evaluated by measuring its 1,1-diphenyl-2-picryl hydrazyl (DPPH), hydrogen peroxide (H2O2), and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) scavenging activity. Also, the metal chelating and reducing power of the fractions was evaluated. Antibacterial activity was determined by the agar well diffusion method against clinical isolates of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Salmonela typhi and the minimum inhibitory concentration (MIC) evaluated by the broth microdilution technique. In the antioxidant assay, the alkaloid fractions exhibited significant antioxidant activity. The root alkaloid fraction exhibited pronounced DPPH, ABTS radical scavenging activity and metal chelating potential with IC50 values of 19.34, 26.02 and 12.01 µg/mL, respectively. The stem alkaloid fraction exhibited high H2O2 scavenging activity (IC50 = 17.46 µg/mL), while the leaf alkaloid fraction demonstrated the optimum reducing power (IC50 = 20.04 µg/mL). The alkaloid fractions exerted remarkable antibacterial activity against the test organisms and MIC values ranged from 0.09 to 2.28 mg/mL, with S. aureus and B. subtilis as the most susceptible. The results highlighted that alkaloid fractions from T. hirtum may serve as natural sources of antioxidant and antibacterial leads and lend support to its use in traditional medicine.

Keywords: Tristemma hirtum, alkaloid fractions, antioxidant activity, antimicrobial activity.
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