DNA Barcoding of Clerodendrum capitatum Using rbcL Gene

Abdulrahman Adamu*, Abdulmumin Z. Abubakar, Adamu A. Ambi, Najma Ilyas, Mujtaba S. Abubakar
Department of Pharmacognosy and drug development, Ahmadu Bello University, Zaria, Kaduna state, Nigeria.
Corresponding Author: ababdulrahman@abu.edu.ng; Tel: +2348065539723
Recieved Date: January 03, 2020; Accepted Date: April 27, 2020; Published Date: 30 April 2020
Citation: Adamu A, Abubakar AZ, Ambi AA, Ilyas N, Abubakar MS. DNA Barcoding of Clerodendrum capitatum Using rbcL Gene.  Trop J Nat Prod Res. 2020; 4(4):123-130. https://doi.org/10.26538/tjnpr/v4i4.2
Copyright: © 2020 Adamu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Clerodendrum capitatum (Willd.) Schum. and Thonn (family: Verbenaceae) is a plant commonly found in the West African region and is widely cultivated in several places in the world. The secondary metabolites of this plant which include but not limited to cyclopeptide alkaloids, triterpenoids, flavonoids, saponins, glycosides, tannins, etc, have health benefits and use in the food and cosmetic industries. The study aims at developing a DNA barcode for Clerodendrum capitatum using standard fragment of rbcL gene. The PCR product was purified and sequenced. Universal rbcL primer pairs were used to amplify a 545bp DNA fragment. A novel sequence (barcode) was deposited in the GenBank database with accession number MK483341 and Protein sequence ID: "QEG53725”. The BLAST search showed significant alignments (99 -100%) with the sequence from Clerodendrum capitatum. It revealed a 100% identity with C. cephalanthum, C. splenden, C. buchneri and 99% identity with Volkameria inermis, C. thomsoniae, C. infortunatum, C. quadriloculare, C. indicum. It showed 6 nucleotides difference with C. wallichi and C. phillipine and 3 nucleotides difference with C. floribundum. The phylogenetic tree reconstructed showed C. capitatum to be monophyletic. The results clearly showed that the use of these conserved DNA sequence as barcode would be an accurate way for species identification and discrimination; this would be useful in medicinal industry to establish DNA based identification of different medicinal plant species to monitor adulteration.

Keywords: Clerodendrum capitatum, Verbenaceae, DNA barcode, rbcL.
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