Antioxidant Effect of Ex-maradi Okra Fruit Variety (Abelmuscus esculentus) on Alloxan-Induced Diabetic Rats

Abdullahi Y. Abbas1*, Ismail Muhammad2, Muhammad B. AbdulRahman3,  Lawal S. Bilbis1
1Department of Biochemistry, Faculty of Science, Usmanu Danfodiyo University, P.M.B. 2346,  Sokoto, Nigeria.
2Department of Biochemistry, Faculty of Natural and applied Sciences, Umaru Musa Yaradua   University, P.M.B. 2218, Katsina, Nigeria.
3Department of Chemical Pathology and Immunology, Faculty of Basic Medicine, College of  Health sciences, Usmanu Danfodiyo University P.M.B. 2346 Sokoto, Nigeria.

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Recieved Date: February 28, 2020; Accepted Date: March 28, 2020; Published Date: 30 March 2020
Citation: Abbas AY, Muhammad I, AbdulRahman MB, Bilbis LS. Antioxidant Effect of Ex-maradi Okra Fruit Variety (Abelmuscus esculentus) on Alloxan-Induced Diabetic Rats. Trop J Nat Prod Res. 2020; 4(3):105-112.
Copyright: © 2020 Abbas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Oxidative stress is associated with the development of diabetic complications and supplementation with antioxidants might delay the development of these complications in diabetic patients. The aim of the present study was to investigate the antioxidant potentials of different parts (Whole Okra, ‘WO’ Okra Peel ‘OP’ and Okra Seed ‘OS’) of Ex-maradi Okra fruit variety on Alloxan-induced diabetic rats. Vitamins A, C and E were determined by spectrophotometric method. Catalase activity was assayed using chemical reactivity method of Beers and Sizer and glutathione by the method described by Petterson and Lazarow. Malonaldehyde (MDA) was measured by the method of Shah and Walker. For antioxidant minerals, Atomic absorption spectroscopy (AAS) was used. Significant (P < 0.05) reduction  in serum levels of vitamins A, C and E, Catalase activity, Reduced Glutathione, Zn, Cr and Mn and significant (P < 0.05) increase in serum levels of Cu, Fe and lipid peroxidation product (MDA) were observed in the diabetic untreated rats compared to the normal control rats.  Oral administration of the different parts of the Okra fruit each at  doses of 100, 200 and 300 mg/kg to the  diabetic rats for 21 days resulted in significant (P < 0.05) reversal of the observed altered aforementioned biochemical parameters in a dose-dependent manner compared to the normal  control rats. The results indicated that the Whole Okra fruit and Okra Seeds of Ex-maradi Okra fruit variety exhibited significant antioxidant effect in Alloxan-induced diabetic rats. 

Diabetes Mellitus, Oxidative stress, Antioxidants, Abelmuscus escuientus, Medicinal plants

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