Ameliorative Effects of D-3-O-Methyl-chiroinositol in Acute and Sub-Chronic Cadmium Chloride Induced Hepatotoxicity

Edwin A. Uwagie-Ero1 and Chinaka O. Nwaehujor 2
1Department of Surgery, Faculty of Veterinary Medicine, University of Benin, Benin City, Nigeria.
2Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B. 1115, Calabar, Nigeria.


Corresponding Author: chinaka_n@yahoo.com; Tel: +2348035450300
Recieved Date: January 23, 2020; Accepted Date: March 26, 2020; Published Date: 30 March 2020
Citation: Uwagie-Ero EA and Nwaehujor CO. Ameliorative Effects of D-3-O-Methyl-chiroinositol in Acute and Sub-Chronic Cadmium Chloride Induced Hepatotoxicity. Trop J Nat Prod Res. 2020; 4(3):80-84.  https://doi.org/10.26538/tjnpr/v4i3.4
Copyright: © 2020 Uwagie-Ero and Nwaehujor. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

D-3-O-Methyl-chiroinositol (D3O), isolated from the stem bark of Piliostigma thonningii has a structural formula similar to phosphatidylinositol phosphate, which participates in the insulin signaling pathways that stimulate glucose transport, and is known to possess strong antioxidant activities. The purpose of the present study was to determine the ameliorative effects of D-3-O-Methyl-chiroinositol on acute and sub-chronic CdCl2-induced hepatotoxicity. Twenty-four rats were assigned to three treatment groups (n = 8). Group A (2 mL distilled water), group B: (2.5 mg/kg b.w. CdCl2 only) and group C: (2.5 mg/kg b.w. CdCl2 and D-3-O-methyl-chiroinositol, 2 mg/kg b.w.). Treatment was daily. Blood was collected from 4 animals per group and analyzed for liver enzymes and a liver section was excised for histopathological analysis at the end of months 1 and 2. The results showed a significant decrease in body and liver weights of the cadmium chloride only challenged group and an increase in the body and liver weights in the D3O-treated and normal groups. D3O significantly ameliorated (p<0.05) the effects of CdCl2 on liver enzymes as seen in the elevated levels of AST, ALP and ALT when compared to CdCl2 only-challenged group. Histopathological studies revealed ameliorative and proliferative (revival) changes in the liver after 60 days of treatment. The administration of D-3-O-methyl-chiroinositol provided a significant protection against CdCl2-induced hepatotoxicity and initiated proliferation of liver cells.

Keywords: D-3-O-Methyl-chiroinositol (D3O), Piliostigma thonningii, Cadmium chloride (CdCl2), Hepatotoxicity, Liver enzymes.
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