Anticancer Activity of Hispidulin from Saussurea simpsoniana and Lupeol from Vincetoxicum arnottianum

Ghulam Hassan1, Qandeel Zahra1, Iftikhar Ali1,2*, Nadja Engel3, Kiran Ali4, Gul Bahreen1, Viqar Uddin Ahmad4, Shahid Aziz2,5, Daijie Wang2
1Department of Chemistry, Karakoram International University, 15100-Gilgit, Gilgit-Baltistan, Pakistan.
2Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan, Shandong Province (250014),  P.R. China.
3Department of Oral and Maxillofacial Surgery, Facial Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany.
4HEJ Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan.
5Department of Chemistry, Mirpur University of Science and Technology (MUST), Mirpur-10250 (AJK), Pakistan.


Corresponding Author: iftikharpcr@yahoo.com; Tel: +92-3339665739
Recieved Date: November 25, 2019; Accepted Date: February 23, 2020; Published Date: 29 February 2020
Citation: Hassan G, Zahra Q, Ali I, Engel N, Ali K, Bahreen G, Ahmad VU, Aziz S, Wang D. Anticancer Activity of Hispidulin from Saussurea simpsoniana and Lupeol from Vincetoxicum arnottianum. Trop J Nat Prod Res. 2020; 4(2):31-35.  doi.org/10.26538/tjnpr/v4i2.2
Copyright: © 2020 Hassan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Saussurea simpsoniana (Field & Gard.) Lipsch (Asteraceae) is a herb, locally known as Boshi Phonar in Gilgit, Pakistan. This herb is used for the treatment of asthma, cough, pneumonia, fever, snakebite, and dysmenorrhoea. The plant Vincetoxicum arnottianum (Wight) Wight (Syn: Cyanchum arnottianum Wight), (Apocynaceae) is used for the treatment of asthma, gastritis, malaria, cholera, skin diseases, wounds, ulcer and constipation. It has recently been reported for its antitumor activity. The present study was aimed to evaluate the methanol extracts of the plants species; S. simpsoniana and V. arnottianum for isolation of the phytoconstituents. As a result of our study, ?-sitosterol, hispidulin and ?-sitosterol-?-D-glucoside were isolated and characterized from S. simpsoniana. While ?-sitosterol, ?-sitosterol-?-D-glucoside, and lupeol were isolated and characterized from V. arnottianum.  Hispidulin and lupeol were tested against breast cancer MCF-7, osteosarcoma MG-63, pancreatic PACA-44, and alveolar rhabdomyosarcoma Rh30 cell lines and hispidulin showed a moderate reduction of cell viability in the range of 20 to 50%.

Keywords: Antiasthmatic herbs, Isolation, Antitumor activity, Metabolic viability assay.
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