Isolation and Characterization of Pyropheophorbide-a from Moringa oleifera Lam

Ukachi E. Igbo1*, John O. Igoli2, 4, Samuel O. Onyiriuka3, Cynthia E. Ogukwe3, Atu A. Ayuk3, Alexander I. Gray2
1Federal Institute of Industrial Research, Oshodi, P.M.B.21023 Ikeja, Lagos, Nigeria. 
2Strathclyde Institute of Pharmacy and Biomedical Sciences. University of Strathclyde, 161 Cathedral Street, Glasgow G4 ORE, Scotland.
3Department of Chemistry, Federal University of Technology, Owerri, Nigeria.
4Department of Chemistry, University of Agriculture, Makurdi, Nigeria.


Corresponding Author: ukachiigbo@yahoo.com; Tel: +234 7064771788
Recieved Date: September 20, 2019; Accepted Date: December 10, 2019; Published Date: 10 December 2019
Citation: Igbo UE, Igoli JO, Onyiriuka SO, Ogukwe CE, Ayuk AA, Gray AI. Isolation and Characterization of Pyropheophorbide a from Moringa oleifera Lam. Trop J Nat Prod Res. 2019; 3(10): 314-318.  https://doi.org/10.26538/tjnpr/v3i10.3
Copyright: © 2019 Igbo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Moringa oleifera is a plant rich in pharmacologically active compounds. This study investigated the phytochemical constituents of Moringa oleifera leaf extracts. Dried M. oleifera leaves were ground and extracted successively with hexane, ethyl acetate and methanol using a Soxhlet apparatus. Different chromatographic techniques were used to fractionate the ethyl acetate extract. Thin layer chromatography was used to pool similar fractions together. Fractions obtained were purified using sephadex glass column. The structures of isolated compounds were identified using nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS) and comparison with published data. Phytochemical screening revealed the presence of flavonoids, terpenoids, carbohydrates and phenols. Ethyl acetate extract which was subjected to column chromatography resulted to the isolation of two compounds: mono acetyl glycerol and pyropheophorbide-a, from the leaves of M. oleifera. This is the first report on the isolation of pyropheophorbide-a from the leaves of M. oleifera.

Keywords: Moringa oleifera, Isolation, Pyropheophorbide-a, Monoacetyl glycerol.
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