Evaluation of The Experimental Spasmolytic and Antiulcer Effect of The Fruit Extract and Fractions of Cucumismetuliferus

Ebere E. Okpalaeke, Amarachi S. Ihim*, Emmanuel I. Peter, Nneoma M. Ofokansi, Sylvester C. Nworu
Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria.
Corresponding Author: ihim.stella@unn.edu.ng; Tel: +2348039576748
Recieved Date: March 05, 2019; Accepted Date: July 04, 2019; Published Date: 09 July 2019
Citation: Okpalaeke EE, Ihim AS, Peter EI, Ofokansi NM,Nworu SC. Evaluation of The Experimental Spasmolytic and Antiulcer Effect of The Fruit Extract and Fractions of Cucumis metuliferus. Trop J Nat Prod Res. 2019; 3(6):195-200.  https://doi.org/10.26538/tjnpr/v3i6.3
Copyright: © 2019 . Okpalaeke et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

The plant Cucumis metuliferus (Cucurbitaceae) locally called ‘bùurarzaàki’ is used by traditional medical practitioners in parts of Plateau state of Nigeria to treat disease such as peptic ulcer, diabetes mellitus, hypertension and HIV/AIDS. This study was undertaken to give credence to the traditional use of Cucumis metuliferus in the treatment of peptic ulcers. The methanol extract of the fruit plant material was fractionated sequentially using n-hexane, ethyl acetate, butanol and water to yield HFCM, EAFCM, BFCM and WFCM fractions, respectively and evaluated for antiulcer properties using experimentally-induced ulcers in mice and rats. In vitro antiulcer activity was also evaluated. Ethanol-induced ulcer was significantly (p<0.05) protected by MECM (100, 200 and 400 mg/kg). The extracts MECM (100, 200 and 400 mg/kg), EAFCM (200, 400 mg/kg), and WFCM (200, 400 mg/kg) exhibited dose-dependent and significant (p<0.05) protection of rats against indomethacin-induced ulcers. Gastrointestinal propulsion in mice was significantly (p<0.05) reduced in a dose-dependent manner with MECM (400 mg/kg) and WFCM (400 mg/kg) showing the highest level of antiperistaltic activity. The contractions evoked by acetylcholine and histamine on the rabbit jejunum were antagonized by the extracts and fractions in a concentration-dependent antispasmolytic manner. Acute toxicity tests showed an oral LD50 greater than 5000 mg/kg in mice. Rich varieties of bioactive constituents in MECM include alkaloids, terpenoids, flavonoids, carbohydrates and steroids. These findings demonstrated that the plant possesses pharmacological properties which lend credence to its ethnomedicinal use as an antiulcer and antidiarrhoeal agent.

Keywords: Cucumis metuliferus, peptic ulcer, antispasmolytic, antiulcer.
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