Extract of Costus spectabilis Attenuates H2O2-Induced Cataract in Cultured Rat Lenses

Salisu Shehu1*, Umar F. Shehu1, Sani Shehu2, Umar H. Danmalam1, Nuhu M. Danjuma3
1Department of Pharmacognosy and Drug Development, Ahmadu Bello University Zaria, Nigeria.
2Department of Pharmacognosy and Drug Development, Kaduna State University, Nigeria.
3Department of Pharmacology and Therapeutics, Ahmadu Bello University Zaria, Nigeria.
Corresponding Author: shehus3621@yahoo.com; Tel: +234-08134924454
Recieved Date: April 08, 2019; Accepted Date: May 06, 2019; Published Date: 07 May 2019
Citation:  Shehu S, Shehu UF, Shehu S, Danmalam UH, Danjuma NM. Extract of Costus spectabilis Attenuates H2O2-Induced Cataract in Cultured Rat Lenses. Trop J Nat Prod Res. 2019; 3(4):145-148. https://doi.org/10.26538/tjnpr/v3i4.7
Copyright: © 2019 Shehu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Ethno-medicinally, fresh sap from the rhizome of Costus spectabilis (CS) or its water extract has been used as eye drop in the management of cataract. The present study is aimed at verifying this claim using H2O2-induced cataract model on isolated, cultured rat lenses. The phytochemical constituents of the extract were verified according to standard methods. Isolated lenses from adult Sprague-Dawley albino rats were cultured in 24-well plates and treated with or without 0.5 mM of H2O2, 0.5 mM of H2O2 plus 0.5 mg/mL, 1 mg/mL, or 2 mg/mL of CS for 24 hrs. Cataract or lens opacity was assessed by scoring and measurement of pixel intensity. Total protein (TPC), Reduced glutathione (GSH), Superoxide Dismutase (SOD) and Malondialdehyde (MDA) level or activity in lens homogenate was measured. Hydrogen peroxide 0.5 mM induced obvious opacity of the lens, but 0.5 mg/mL of CS significantly (P ? 0.001) decreased it. Other doses of CS demonstrated mild decrease in cataract formation or opacity of lens. Furthermore, 0.5 mM of H2O2 caused significant decrease (P ? 0.05) of TPC, GSH and increase of MDA level in the lens, but this has not been significantly reversed by CS (0.5-2 mg/ml). The identified phytochemical constituents of the extract include; Steroids, Triterpenes, saponins and flavonoids. The observed activity of the extract may be attributed to the presence of one or some of these constituents.

Keywords: Cataract, Opacity of lens, Pixel intensity, Phytochemical constituent.
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