DNA and HPLC Fingerprint of Commiphora africana (Burseraceae)

Abdulmumin Z. Abubakar*, Zainab Mohammed, Abdulrahman Adamu, Umar F. Shehu, Aliyu Nuhu, Salisu Shehu, Garba Ibrahim, Mujitaba S. Abubakar
Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria, Nigeria.  
Corresponding Author: azabubakar01@gmail.com; azabubakar@abu.edu.ng; Tel: +2348065904149
Recieved Date: January 15, 2019; Accepted Date: February 18, 2019; Published Date: 01 March 2019
Citation: Abubakar AZ, Mohammed Z, Adamu A, Shehu UF, Nuhu A, Shehu S, Ibrahim G, Abubakar MS. DNA and HPLC Fingerprint of Commiphora africana (Burseraceae).  Trop J Nat Prod Res. 2019; 3(2):37-41.  https://doi.org//10.26538/tjnpr/v3i2.3
Copyright: © 2019 Abubakar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Commiphora africana is a famous medicinal plant used traditionally in the treatment of various ailments such as inflammation, stomach disorders, diabetes, malaria, tumours, skin infections and as antidotes for venomous bites and stings in Nigeria and other parts of Africa. The present study aims to evaluate the molecular and chemical properties that could be of value in the standardization and quality assessment of the medicinal values of C. africana. The randomly amplified polymorphic deoxyribonucleic acid – polymerase chain reaction (RAPD - PCR) analysis was carried out on the leaf sample. The high-performance liquid chromatography (HPLC) was carried out on the leaf and barks of stem and root extracts. The RAPD-PCR amplified products generated fingerprint patterns ranging from 250 to 1600 base pairs. The oligoprimers (A, B and E) produced distinct banding patterns for C. africana, the num¬ber of well-defined and major bands for a single primer ranged from 2 to 7 with maximum number of well-defined bands ob¬served with primer ‘a’ (7 bands) and the minimum number with primer ‘e’ (2 bands). The HPLC chromatograms of the extracts of leaf and barks of stem and root of C. africana showed some similarities and differences in the type, number and concentration of the compounds in the various morphological parts of the plant. The DNA and HPLC fingerprints obtained from this research can be useful in the identification and authentication of C. Africana and thus may be useful in the standardization of this plant.

Keywords: Medicinal plant, C. africana, HPLC, RAPD.
Back to Articles