Cytotoxic Activity of Croton penduliflorus (Euphorbiaceae) Seed Oil against Ehrlich Ascites Carcinoma Cells

Abiola S. Ojokuku1*, Omolola S. Odesanmi2, Abiodun R. Lawal2, Abiola O. Magbagbeola2, Mehmet D. Ozaslan3, Didem I. Karagoz3, Halil I. Kilic3, Izzetin Guler3
1Department of Chemical Science, Yaba College of Technology, P.M.B. 2011 Yaba, Lagos, Nigeria.
2Department of Biochemistry, College of Medicine, University of Lagos, P.M.B. 12003 Lagos, Nigeria.
3Department of Biology, University of Gaziantep, 27310 Gaziantep, Turkey.

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Recieved Date: June 20, 2018; Accepted Date: December 07, 2018; Published Date: 03 January 2019
Citation: Ojokuku AS, Odesanmi OS, Lawal AR, Magbagbeola AO, Ozaslan MD, Karagoz DI, Kilic HI, Guler I. Cytotoxic Activity of Croton penduliflorus (Euphorbiaceae) Seed Oil Against Ehrlich Ascites Carcinoma Cells. Trop J Nat Prod Res. 2018; 2(12):498-501.
Copyright: © 2018 Ojokuku et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Croton penduliforus is a tropical medicinal plant ubiquitous to Nigeria and other West African countries. Its seeds are used in folklore medicine to treat uterine tumour and fibroid. This study was carried out to determine the cytotoxic effect of Croton penduliforus seed oil (CPSO) on Ehrlich ascites carcinoma (EAC). The shelled, oven-dried, ground seeds of Croton penduliforus was extracted with petroleum ether using Soxhlet apparatus to obtain the oil. Cytotoxicity of CPSO on EAC were carried out using trypan blue dye exclusion assay. A stock solution of 1000 µg/mL of CPSO in Dimethyl sulphoxide (DMSO) was prepared followed by serial dilutions of 100, 10 and 1 µg/mL from the stock. Aliquots of 200 µL of CPSO sample were placed in the wells and the volume were made up to 800 µL with DMSO. Aliquot of 100 µL of EAC with a concentration of 106 cells/mL was added to each well. Negative control having 800 µL DMSO in the wells followed by the addition of 100 µL of EAC with a concentration of 106 cells/mL were also prepared, while 5-Fluorouracil (5-FU) with concentrations of 1000, 100, 10 and 1 µg/mL was used as the positive control. The wells were incubated at 37oC for 24 h. Following the incubation, 100 µL of trypan blue was added to each well. The number of viable cells were counted in each well. CPSO has low cytotoxic effect on EAC with IC50 of 561 µg/mL, while 5-FU has IC50 of 2.88 µg/mL.

Keywords: Croton penduliforus, Extract, Ehrlich Ascites Carcinoma, cytotoxicity.
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