Antioxidant and ?-Glucosidase Inhibitory Activities of Phenolic Compounds Isolated from the Aerial Parts of Ampelocissus africanus

Lukman Mustapha1, Ezekiel Garba1, Umar U. Pateh2, Bilqis A. Lawal3, Bolatito E. Olanipekun4, Florence Tarfa5, Augustine A. Ahmadu6*
1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna-Nigeria
2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria-Nigeria
3Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, University of Ilorin, Ilorin-Nigeria
4Department of Chemical, Geological and Physical Sciences, Faculty of Science, Kwara State University, Malete, Nigeria
5Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development (NIPRD),Idu-Abuja, Nigeria.
6Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Calabar, Calabar-Nigeria

Corresponding Author: ahmadu2001@yahoo.com; Tel: +234 8037033505
Recieved Date: 22 July 2021; Accepted Date: 19 September 2021; Published Date: 4 October
Citation: Mustapha L, Garba EE, Pateh UU, Lawal BA, Olanipekun BE, Tarfa F, Ahmadu AA. Antioxidant and α-Glucosidase Inhibitory Activities of Phenolic Compounds Isolated from the Aerial Parts of Ampelocissus africanus. Trop J Nat Prod Res. 2021; 5(9):1683-1687. http://www.doi.org/10.26538/tjnpr/v5i9.25
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© 2021 Mustapha et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Ampelocissus africanus is an important medicinal plant with diverse ethnomedicinal uses. The purpose of this study was to investigate the antioxidant and ?-glucosidase inhibitory activities of phenolic compounds isolated from the aerial parts of Ampelocissus africanus.Two known compounds were isolated using a combination ofcolumn and thin layer chromatography. The structures were elucidated usingUV and NMR spectroscopy. The antioxidant activity of the compounds was evaluated using 2,21-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays, while the antidiabetic potential was investigated using the ?-glucosidase inhibition assay. Ascorbic acid and acarbose were used as standard and data obtained were expressed as mean ± SEM.The IC50 of the two compounds were calculated using a suitable regression analysis and graph pad prism software 6.0 using t-test.Compounds 1 and 2 were isolated as a yellow solids and identified as methyl gallate and quercetin, respectively. Result of the antioxidant assay using ABTS revealed that compound 1 gave a percentage inhibition of 98.22±0.79, while compound 2 gave 99.04±0.39% inhibition. The DPPH assay revealed inhibition at 59.38±3.23% and 63.45±2.56% for compounds I and 2, respectively. The values for DPPH assay were significantly different at p value <0.05 when compared to ascorbic acid. Compound 2 exhibited ?-glucosidase inhibitory activity (IC50 4.02±0.37 ?g/mL) though lower than acarbose (IC50 0.98± 0.57 ?g/mL). Compound 1 however, showed weak ?-glucosidase inhibitory activity with IC50>100µg/mL. These results might justify the ethnomedicinal uses of this plant in ameliorating metabolic disorder such as diabetes.

Keywords: Ampelocissus africanus, ?-glucosidase, Antioxidant assays, Quercetin, Methyl gallate
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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