Ameliorative Effects of Monoclonal Antibodies (CD3 and PCNA) on the Immunomodulation of Mesenchymal Stem Cells in Male Mice

Nabil A Soliman1*, Heba M Saad El-Dien2, Aya Hussin1
1Department of Zoology, Faculty of Science, Zagazig University, Egypt
2Tissue Culture and Molecular Biology Center, Faculty of Medicine, Assuit University, Egypt









Corresponding Author: nabilsoliman5419@yahoo.com; Tel: 00201096380800
Recieved Date: 21 November 2020; Accepted Date: 25 September 2021; Published Date: 4 October
Citation: Soliman NA, El-Dien HMS, Aya Hussin A. Ameliorative Effects of Monoclonal Antibodies (CD3 and PCNA) on the Immunomodulation of Mesenchymal Stem Cells in Male Mice. Trop J Nat Prod Res, 2021; 5(9):1626-1633. http://www.doi.org/10.26538/tjnpr/v5i9.16
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© 2021 Soliman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Mesenchymal stem cell (MSC) is being used in regenerative medicine and also for the treatment of autoimmune diseases and graft-versus-host disease. Cyclophosphamide (CTX) is a chemotherapeutic agent used for the treatment of cancer as well as several immunological diseases. The present study was designed to evaluate the therapeutic role of bone marrow-derived mesenchymal stem cells (BM-MSCs) in ameliorating the adverse effect of cyclophosphamide-induced immunohistochemical toxicity in adult male albino mice. Forty male albino mice of matched age were used for this study. They were divided into four equal groups. Group I served as the control; Group II was treated with cyclophosphamide (CTX); Group III was administered with cyclophosphamide concomitantly with MSCs; while the fourth group was used to isolate bone marrow. Blood leukocyte, splenocyte numbers, blood cell type levels, and splenocyte were determined after one month of initiation of the experiment. Two markers; the cluster of differentiation 3 (CD3) and proliferating cell nuclear antigen (PCNA) were used for immunohistochemical analysis. The results showed that CTX induced a reduction in overall proliferating cells in splenic parenchyma with clear CD3 positive cells in the periarterial sheath. MSCs induced reduction in T-cell number sparing other cells as indicated from intense cell proliferation in the splenic white and red pulp in this group of animals. The study demonstrated that MSCs induced reduction of T-cell number associated with a proliferation of other immune cells of the spleen.

Keywords: Cluster of differentiation, Cyclophosphamide, Mesenchymal stem cell, Proliferating cell nuclear antigen, T-cells.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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