Effect of Pre-soaking Mustard Green Seeds Prior to Cold Plasma Treatment on Bioactive Aspects of Microgreens

Khanit Matra1, Worachot Saengha2, Yottana Tanakaran1, Thipphiya Karirat2, Benjaporn Buranrat3, Sirirat Deeseenthum2, Vijitra Luang-In2*
1Department of Electrical Engineering, Faculty of Engineering, Srinakharinwirot University, Nakhon Nayok 26120, Thailand.
2Natural Antioxidant Innovation Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.
3Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand.

Corresponding Author: vijitra.l@msu.ac.th ; Tel: +66 (0)43754085 ext. 1833
Recieved Date: 03 June; Accepted Date: 15 August ; Published Date: 03 September
Citation: Matra K, Saengha W, Tanakaran Y, Karirat T, Buranrat B, Deeseenthum S, Luang-In V. Effect of Pre-soaking Mustard Green Seeds Prior to Cold Plasma Treatment on Bioactive Aspects of Microgreens. Trop J Nat Prod Res. 2021; 5(8):1422-1426. http://www.doi.org/10.26538/tjnpr/v5i8.16
© 2021 Matra et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Microgreens have been reported to contain higher phytochemicals than mature plants. Mustard green (MG) is rich in glucosinolates that produce isothiocyanates (ITCs) upon degradation. ITCs have well-known chemopreventive benefits. Previously, non-thermal plasma treatment on MG seeds could enhance bioactive content; However, seed pretreatment before non-thermal plasma treatment was yet to be studied. The aim of this work was to evaluate the impact of pre-soaking MG seeds overnight before non-thermal plasma treatment at 21 kV for 5 min on phytochemicals and activities of MG microgreens. Plasma-treated pre-soaked (PTPS) seeds and control seeds (no plasma treatment) received deionized water for 7 days. No difference in germination percentage, stem height or fresh/dried weight of MG between PTPS seeds and the control was observed. Total ITC content in MG microgreens from PTPS seeds (1.62 mmol/100 g DW) was significantly higher than control seeds (1.00 mmol/100 g DW). Antioxidant capacity and total phenolic/flavonoid compounds were similar in both MG microgreens. Lower IC50 values were recorded for MG microgreens from PTPS seeds at most time intervals, indicating higher cytotoxicity against MCF-7 and HepG2, and greater effectiveness in cancer proliferation inhibition than the control. No differences in Bax, Caspase-3 and Bcl-2 mRNA expressions were detected for all samples; however, MMP-9 mRNA expression was reduced in MG microgreens from PTPS seeds for HepG2. Pre-soaking MG seeds before plasma treatment at 21 kV for 5 min increased ITC levels and increased anticancer activities of MG microgreens without adverse effects compared to MG microgreens from control seeds.

Keywords: Plasma, HepG2, MCF-7, Mustard green, Soaking.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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