Isolation and Characterization of Bacteria with Multiple Drug Resistance from Pig Dung

John O. Agboola1,2, Abiodun A. Ayandele1, John A. Amao3*

1Department of Pure and Applied Biology, LAUTECH, Ogbomoso, Nigeria
2Forestry Based Rural Resource Centre, Ikeja, Nigeria
3Department of Microbiology, University of Ilorin, Ilorin, Nigeria

Corresponding Author: ayoamao@gmail.com; Tel: +2347032328461
Recieved Date: 17 June 2021; Accepted Date: 25 August; Published Date: 03 September
Citation: Agboola JO, Ayandele AA, Amao JA. Isolation and Characterization of Bacteria with Multiple Drug Resistance from Pig Dung. Trop J Nat Prod Res. 2021; 5(8):1506-1514. http://www.doi.org/10.26538/tjnpr/v5i8.29
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© 2021 Agboola et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Large quantities of antibiotics are used in agriculture, predominantly in animal farming; this has steered the problem of antibiotics resistance in bacteria. This study was aimed at determining the antibiotic resistance pattern of bacterial isolates from pig dung. Large quantities of antibiotics are used in agriculture, predominantly in animal farming; this has steered the problem of antibiotics resistance in bacteria. The most troublesome issue is that resistance can increase with incessant and widespread usage of antibiotics. Pig farms, known to employ the use of antibiotics were considered for sampling, 30 pig dung samples from five pig farms in Ogbomoso were collected using sterile spatula into sampling tubes. Antibiotics used were ceftriaxone (30 µg), cefepime (30 µg), cefixime (5 µg), ceftazidime (30 µg), cefuroxime (30 µg), gentamicin (10 µg), cefixime (5 µg), ofloxacin (5 µg), augmentin (30 µg), nitrofurantoin (300 µg) and ciprofloxacin (5 µg), all products of Oxoid. The isolates were all resistant to nine of the 10 antibiotics tested, but they all had an intermediate susceptibility pattern to Cefepime. A high percentage (97.35%) of resistance were observed for cefixime, whereas the rate of resistance to cefuroxime was 24.78%. Four of the isolates were identified as Alcaligenes faecalis while three were identified as Achromobacter denitrificans. The results of this study have revealed an emerging resistance to antimicrobial drugs among bacteria species from pig dung in Ogbomoso; and this may result into serious public health problems as the resistant bacteria make their passages to human populations.

Keywords: Alcaligenes, Antimicrobial, Bacteria, Pig dung, Resistance.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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