Evaluation of Antioxidant Activity and Cytotoxic Potential of Thymus vulgaris Leaf Extracts

Neran A. Thamer1*, Alyaa H. Hammadi2, Muna Mohammed Yaseen3
1Medical Technical College, Al-Farahidi University, Al-Jadiriyah Bridge, Baghdad, Iraq
2Department of Health, Ministry of Health, Diwaniyah, Diwaniyah Teaching Hospital, Diwaniyah, Iraq
3Department of Basic Science, College of Dentistry, University of Anbar, Al-Anbar, Iraq

Corresponding Author: neran.technical@gmail.com; Tel: 00964 7730208558
Recieved Date: 07 April 2021; Accepted Date: 11 August 2021; Published Date: 03 September
Citation: Thamer NA, Hammadi AH, Yaseen MM. Evaluation of Antioxidant Activity and Cytotoxic Potential of Thymus vulgaris Leaf Extracts. Trop J Nat Prod Res. 2021; 5(8):1389-1396. http://www.doi.org/10.26538/tjnpr/v5i8.11
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© 2021 Thamer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Thymus vulgaris is a most important medicinal plant, it is rich in bioactive secondary metabolites. The leaves are used for the treatment of several diseases. In the present study, the antioxidant activity and cytotoxic potential of T. vulgaris leaf extracts were evaluated. Leaf extracts were prepared using cold and hot procedures with varied polarity solvents (chloroform, acetone, ethanol, and aqueous methanol [50% v/v]). The total phenol content (TPC) and antioxidant potential of the extracts were determined. The leaf extracts were fractionated using Gas Chromatography/Mass Spectroscopy (GC/MS), and the acetone extracts cytotoxic effects on breast cancer cell lines were assessed. The results indicated that the aqueous methanol extract (hot method) had the highest TPC (76.45 ± 2.88 mg/g of dry plant content of gallic acid equivalence [mgGAEg-1]), while the lowest TPC was obtained in the chloroform extract (cold method; 37.36 ± 2.45 mgGAEg-1). The maximum antioxidant activity was observed in the ethanol extract (hot method; 83.9% ± 0.96), while a minimum value was recorded for the acetone extract (hot; 66.3% ± 0.71) at a concentration of 100 ?g/mL. GC/MS analysis revealed the presence of phenolic compounds such as p-Cymen-3-ol, p-Cymen-2-ol, 2,5-diethylphenol, 6-ethyl-3, 4-dimethylphenol, 7,11-dimethyldodeca-2,6,10-trien-1-ol thymol and some flavonoids. The acetone extract showed an anticancer effect on the MCF-7 and Cal-51 breast cancer cell lines, as well as the HBL-100 normal cell line. The results of this study provide scientific justification for the medicinal use of T. vulgaris leaf extracts that contained various polyphenol components; it has strong antioxidant and antitumor activity.

Keywords: Antioxidant capacity, Cytotoxic activity, GC-MS analysis, Thymus vulgaris.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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