Optimization of Total Flavonoid Content from Cardamom Fruits Using a Simplex-Centroid Design, Along with the Evaluation of the Antioxidant Properties

Waras Nurcholis1,2*, Khusnul Ma’rifah1, Made I. Artika1, Syarifah I. Aisyah3, Bambang P. Priosoeryanto2,4
1Department of Biochemistry, Faculty of Mathematic and Natural Science, IPB University, Bogor, Indonesia
2Tropical Biopharmaca Research Center, IPB University, Bogor, Indonesia
3Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia
4Division of Veterinary Pathology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia

Corresponding Author: wnurcholis@apps.ipb.ac.id; Tel: +62-8179825145
Recieved Date: 05 April 2021; Accepted Date: 10 August 2021; Published Date: 03 September
Citation: Nurcholis W, Ma’rifah K, Artika MI, Aisyah SI, Priosoeryanto BP. Optimization of Total Flavonoid Content from Cardamom Fruits Using a Simplex-Centroid Design, Along with the Evaluation of the Antioxidant Properties. Trop J Nat Prod Res. 2021; 5(8):1382-1388. http://www.doi.org/10.26538/tjnpr/v5i8.10
© 2021 Nurcholis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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The fruits of Cardamom (Amomum compactum Sol. Ex Maton; Zingiberaceae), commonly known as Queen species, which contained flavonoid compounds, have a variety of pharmacological properties and are used in traditional medicine. Therefore, this study aimed to employ solvent macerations to optimize the total flavonoid extraction from Cardamom fruits, as well as evaluate their antioxidant activity. The fruits were collected from a local farmer in Bogor, Indonesia, in August 2019. Then, different extraction solvents, namely water, acetone, methanol, and ethanol, alongside their binary, ternary, and quaternary combinations, were investigated using a simplex-centroid design. The optimized extracts were tested for their flavonoid content using an aluminium chloride reagent and their antioxidant activity compared by utilizing 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. Water extracts showed the maximum flavonoid content of 88.01 mg/g DW with the highest reducing antioxidant activity at 1110.95 ?mol TE/g DW by FRAP assay. Meanwhile, the radical scavenging activity by the DPPH method revealed the highest value for the water-ethanol binary mixture to be 153.17 ?mol TE/g DW. Consequently, the water and water-ethanol are the optimal solvents for extracting cardamom flavonoid compounds with antioxidant properties.

Keywords: Amomum compactum Sol. Ex Maton, DPPH, Flavonoid, FRAP, Mixture designs, Solvent extraction.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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