Antioxidant Activity of Different Parts of Nauclea subdita 

Christina Avanti1, Etis Remanti2, Tegar A. Yuniarta3, Azminah Azminah3, Oeke Yunita2, Finna Setiawan2*

1Department of Pharmaceutics. Faculty of Pharmacy. University of Surabaya (UBAYA). Surabaya 60293. East Java. Indonesia
2Department of Pharmaceutical Biology. Faculty of Pharmacy. University of Surabaya (UBAYA). Surabaya 60293. East Java. Indonesia
3Department of Pharmaceutical Chemistry. Faculty of Pharmacy. University of Surabaya (UBAYA). Surabaya 60293. East Java. Indonesia

Corresponding Author: finna@staff.ubaya.ac.id; Tel: +6281703396622
Recieved Date: 15 March 2021; Accepted Date: 06 August 2021; Published Date: 03 September
Citation: Avanti C, Remanti E, Yuniarta TA, Azminah A, Yunita O, Setiawan F. Antioxidant Activity of Different Parts of Nauclea subdita. Trop J Nat Prod Res. 2021; 5(8):1365-1370. http://www.doi.org/10.26538/tjnpr/v5i8.7
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© 2021 Avanti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Nauclea subdita (Taya) has been used empirically to treat hypertension patients in Kalimantan Island. Recent studies reported that hypertension is very closely related to oxidative stress. Natural antioxidants from plants act as radical scavengers by converting free radicals into less reactive substances. Therefore, a plant possessing antioxidant activity could be a promising agent as an alternative treatment for hypertension. This study aimed to determine the in vitro antioxidant activity of aqueous extract of the stem bark and leaves of Nauclea subdita. Assay methods used in this study were Ferric Reducing Antioxidant Power (FRAP), 2.2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS). Furthermore, a molecular docking study was performed to predict the potential antioxidant activity of several chemical constituents of the plant against protein kinase C. The results indicated that the antioxidant activity of Taya extracts increases linearly with its concentration, as also indicated by DPPH and ABTS assay. According to the ABTS assay, the stem bark and leaves extract possessed antioxidant activity with IC50 values of 1183.13 ppm and 116.42 ppm, respectively and 128.63 ppm and 26.95 ppm for DPPH assay. At the same time, the FRAP assay showed ferric reducing power, which correlates to its antioxidant activity. The Molecular docking study showed that two metabolites from the phytosteroid class (?-sitosterol and stigmast-4-en-3-one) yielded comparable activity with native ligands. Aqueous extract of Taya stem bark and leaves possess antioxidant capacity, which could be further studied as a potential agent to reduce oxidative stress in hypertension.

Keywords: Taya, Antioxidant, DPPH, ABTS, FRAP.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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