Hydrogen-Rich Water Ameliorates Pancreatic Cell Necrosis and Regulates Cytokine Levels in Streptozotocin-Induced Diabetic Rats

Budi Susatia1*, Ekowati Retnaningtyas1, Siti N. Arifah2, Sri R. Lestari2
1Department of Nursing, Politeknik Kesehatan Kemenkes Malang, Jalan Besar Ijen, Malang, East Java, Indonesia
2Department of Biology, Faculty of Mathematics and Natural Sciences, Jl. Semarang, Malang, East Java, Indonesia

Corresponding Author: budi_susatia@poltekkes-malang.ac.id; Tel: +6281235028007
Recieved Date: 14 March 2021; Accepted Date: 12 August 2021; Published Date: 03 September
Citation: Susatia B, Retnaningtyas E, Arifah SN, Lestari SR. Hydrogen-Rich Water Ameliorates Pancreatic Cell Necrosis and Regulates Cytokine Levels in Streptozotocin-Induced Diabetic Rats. Trop J Nat Prod Res. 2021; 5(8):1360-1364. http://www.doi.org/10.26538/tjnpr/v5i8.6
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© 2021 Susatia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Water plays an important role in the metabolic processes. An unhealthy lifestyle leads to an increase in free radicals, which causes type 2 diabetes mellitus (T2DM). Hydrogen-rich water (HW) is water with dissolved hydrogen gas and has been implicated to have antioxidant activity. This study was aimed at analyzing the effect of HW on pancreatic cell necrosis and cytokine level in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were fed a high-fat diet (HFD) for four weeks before being injected intraperitoneally with low dose STZ (35 mg/kg bw) to obtain DM rats. Blood glucose values of >200 mL/dL were considered diabetic rats. The experimental rats were randomly divided into five groups: healthy (normal rats without STZ injection), DM, DM + metformin (45 mg/kg bw), DM + HW, and DM + HW + metformin. The treatments were administered orally for fourteen days. After that, the rats were dissected using Ket-A-Xyl; serum and liver samples were obtained to determine the concentrations of IL-10 and IL-1?. The pancreas was collected and examined for cell damage, while histology of the Islet of Langerhans was analyzed descriptively. The results showed that HW reduced blood glucose levels, body weight, and IL-1? concentration, increased IL-10 concentration, and improved necrotic cells of the pancreas in comparison to DM rats. HW could be one of the therapies for T2DM patients by lowering glucose levels, regulating cytokine levels, and repairing ?-cells in the pancreas.

Keywords: Diabetes mellitus, Hydrogen-rich water, IL-1?, IL-10, Necrosis.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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