Chemical Composition, In vitro Evaluation of Antioxidant Properties and Cytotoxic Activity of the Essential Oil from Calamintha incana (Sm.) Helder (Lamiaceae)

Arwa R. Althaher1,2*, Sawsan A. Oran2, Yasser K. Bustanji3,4,5

1Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, 11733, Amman, Jordan
2Department of Biological Sciences, Faculty of Sciences, The University of Jordan, 11942, Amman, Jordan
3Department of Basic Medical Sciences, College of Medicine, University of Sharjah, 27272, Sharjah, UAE
4Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, The University of Jordan, 11942, Amman, Jordan
5Hamdi Mango Center for Academic Research, The University of Jordan, 11942, Amman, Jordan

Corresponding Author: a.althaher@outlook.com; Tel: +962-6-5355000/22226
Recieved Date: 05 December 2020; Accepted Date: 10 August 2021; Published Date: 03 September
Citation: Althaher AR, Oran SA, Bustanji YK. Chemical Composition, In vitro Evaluation of Antioxidant Properties and Cytotoxic Activity of the Essential Oil from Calamintha incana (Sm.) Helder (Lamiaceae). Trop J Nat Prod Res. 2021; 5(8):1333-1339. http://www.doi.org/10.26538/tjnpr/v5i8.2
Copyright:
© 2021 Althaher et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at  Tropical Journal of Natural Product Research


ABSTRACT

Calamintha incana (Sm.) Helder (Lamiaceae) is an aromatic herb used in folk medicine in Jordan and neighboring countries. This study aimed to isolate and characterize the chemical composition of essential oils extracted from the aerial parts of C. incana and to evaluate the total phenolic and flavonoid contents, antioxidant activity, and cytotoxic potential. The essential oils from the aerial parts of C. incana (CIEO) were extracted by hydrodistillation, and GC/MS were performed for the chemical analysis of the oil. Total phenol and flavonoid contents were assessed using the colorimetric assay. The antioxidant activity of the oil was assessed using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power ability. The cytotoxic activity of CIEO against MCF-7, T47D, Caco-2 cancer cell lines, and normal fibroblast cell line (MRC-5) was investigated by 3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The results demonstrated that the main constituents of CIEO were benzenamine-4-methyl-3-nitro-(34.11%), and (2S,4R)-p-mentha-6,8-diene 2-hydroperoxide (31.48%). The phenolic content of CIEO was higher than the flavonoid content. With respect to the DPPH radical scavenging activity, the IC50 was 15.38 mg/mL, while for the reducing power ability the EC50 was 9.79 mg/mL). Moreover, CIEO was cytotoxic against cancerous and non-cancerous cells at 200 ?g/mL. In conclusion, the essential oil extract of C. incana is characterized by its non-terpenoid aromatic compounds. Phenols were more abundant than flavonoids, and CIEO had a good antioxidant capacity and non-selective cytotoxic activity. Therefore, additional investigations are required to understand the mechanism of the cytotoxicity of this plant.

Keywords: Antioxidant effect, Cytotoxicity, Essential oils, Lamiaceae, Calamintha incana.
Back to Articles

ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
Scopus citescore 0.3 (2020)

Indexing & Abstracting

citescore 0.3 (2020)

j-gate logo

International Innovative Journal Impact Factor

African Index Medicus

CAS

Index Copernicus International

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Crossref Content Registration logo

WorldCat Discovery Service

Geneva Foundation for Medical Education and Research