Molucidine and Desmethylmolucidine as Immunostimulatory Lead Compounds of Morinda lucida (Rubiaceae)

Chigozie C. Ezeagha1*, Daniel L. Ajaghaku2, Eze E. Ajaegbu3, Kenneth G. Ngwoke4, Patience O. Osadebe5, Festus B.C. Okoye4

1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Chukwuemeka Odumegwu Ojukwu University Igbariam, Anambra State, Nigeria
2Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Enugu State, Nigeria
3Pure and Applied Sciences Department, Faculty of Pure and Applied Sciences and General Studies, Federal College of Dental Technology and Therapy, Enugu, Nigeria.
4Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University Awka, Anambra State, Nigeria
5Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria

Corresponding Author: cc.ezeagha@coou.edu.ng; Tel: +2348068583688
Recieved Date: 16 March 2021; Accepted Date: 14 May 2021; Published Date: 03 June
Citation: Ezeagha CC, Ajaghaku DL, Ajaegbu EE, Ngwoke KG, Osadebe PO, Okoye FBC. Molucidine and Desmethylmolucidine as Immunostimulatory Lead Compounds of Morinda lucid (Rubiaceae). Trop J Nat Prod Res. 2021; 5(5):977-982.  http://www.doi.org/10.26538/tjnpr/v5i5.29
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© 2021 Ezeagha et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Morinda lucida is in great demand in ethnomedicine for treating different kinds of sicknesses. This research work was directed towards validation of the immunostimulatory potentials of M. lucida in immunocompromised animal model as well as filling the gap in knowledge on the bioactive compounds responsible for this effect. The methanol leaf extract was separated into n-hexane, ethyl acetate and butanol fractions. The extract and fractions were tested on cyclophosphamide immunosuppressed mice at doses ranging from 50 – 200 mg/kg with levamisole as positive control. The chromatographic methods were used in purification and isolation of the major bioactive compounds and their structures elucidated by 1D NMR spectroscopy. Other minor compounds present in the fractions were determined by dereplication using HPLC-DAD analysis. The isolated Compounds 1 and 2 were elucidated as molucidine and desmethylmolucidine respectively. Other minor compounds present in the fractions were luteolin, kaempferol-3-O-glucoside, quercetin, cinnamic acid, hyperoside, and cyclopenol. The extract and fractions showed significant (p < 0.05) increase in cellular immunity with ethyl acetate fraction being the most active fraction. The identification and isolation of these compounds as lead compounds responsible for the immunostimulatory activity constitute additional knowledge in the medicinal properties of M. lucida.

Keywords: Morinda lucida, Immunostimulatory, Molucidine, Desmethylmolucidine.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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