Production of Biosurfactants Aspergillus niger and Rhodotorula sp Isolated from Sugar Cane Bagasse Dumpsite: A Comparative Study


Ekene J. Nweze1, Chibuike S. Ubani1, Justus N. Nwachukwu1, Amarachukwu V. Arazu2 ⃰, Obinna A. Oje3, Obioma U. Njoku1


1Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
2Department of Science Laboratory Technology, University of Nigeria, Nsukka, Nigeria
3Department of Biochemistry, Federal University, Ndufu-alike, Ikwo, Nigeria



Corresponding Author: vivian.arazu@unn.edu.ng; Tel: +2347039332134
Recieved Date: 30 November 2020; Accepted Date: 19 May 2021; Published Date: 03 June
Citation: Nweze EJ, Ubani CS, Nwachukwu JN, Arazu AV, Oje OA, Njoku OU. Production of Biosurfactants Aspergillus niger and Rhodotorula sp Isolated from Sugar Cane Bagasse Dumpsite: A Comparative Study. Trop J Nat Prod Res. 2021; 5(5):924-927. http://www.doi.org/10.26538/tjnpr/v5i5.20
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© 2021 Nweze et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Biosurfactants are amphiphilic and surface-active compounds produced by microorganisms. Their relevance can be seen in petroleum, agriculture and pharmaceutics. Environmental pollution from agricultural wastes and by products has reached its peak and alternate applications of these wastes for other possible benefits are the current trends. This study compared the physicochemical properties and activity of biosurfactants produced from fungi isolated from sugar cane bagasse dumpsite. Two species of fungi were isolated and identified from sugar cane bagasse and identified as Aspergillus niger and Rhodotorula sp. by physiological and morphological tests. They were grown on mineral salt medium (MSM) for 14 days using sugar cane bagasse as carbon source. The microbial broths were assayed for biosurfactant production using drop collapse method, oil displacement method, and emulsification method. The emulsification activity of biosurfactants produced by Rhodotorula sp. recorded 51.133 ± 4.964% which was significantly different when compared to that of Aspergillus niger (48.067 ± 3.126%). The Aspergillus niger performed poorly in the drop collapse test assay (DCTA). There was a significant difference in the clear zone formation (CZF) produced by Rhodotorula sp (0.167±0.029 cm) when compared to that of the Aspergillus niger (0.133 ± 0.289 cm).  The biosurfactant produced by Rhodotorula sp. had a higher wetting and emulsification activity. This study suggests that the crude biosurfactants isolated from Rhodotorula sp show have a wider application in pharmaceutical industries where emulsion is important towards efficiency in production.

Keywords: Biosurfactant, Micro-organism, Sugar cane Bagasse, Wetting property.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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