Gene Expression Modulation of Apoptotic and Oestrogen Receptor Alpha Genes by Active Fractions of Selected Nigerian Plants on Cervical Cancer Cell Line (HeLa)

Saibu G. Morounke1*, James B. Ayorinde2, Omilabu A. Sunday3, Magbagbeola A. Olubunmi4, Fadaka O. Olawale5, Oguntibeju O. Olufemi6, Meyer Meyer5
1Department of Biochemistry, Lagos State University, Lagos State, Nigeria
2Department of Biochemistry & Nutrition, Nigerian Institute of Medical Research, Yaba, Lagos State, Nigeria
3Central Research Laboratory, College of Medicine-University of Lagos, Lagos State, Nigeria
4Department of Biochemistry, College of Medicine-University of Lagos, Lagos State, Nigeria
5DSI/Mintek Nanotechnology Innovation Centre Biolabels Node Department of Biotechnology Life Science Building, University of the Western Cape, Cape Town, South Africa
6Phytomedicine and Phytochemistry Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville, 7535, South Africa




Corresponding Author: gbemisola.saibu@lasu.edu.ng; Tel: +234-8182184036
Recieved Date: 06 February 2021; Accepted Date: 10 April 2021; Published Date: 03 May
Citation: Gbemisola SM, Ayorinde JB, Sunday OA, Olubunmi MA, Olawale FO, Olufemi OO, Meyer M. Gene Expression Modulation of Apoptotic and Oestrogen Receptor Alpha Genes by Active Fractions of Selected Nigerian Plants on Cervical Cancer Cell Line (HeLa). Trop J Nat Prod Res. 2021; 5(4): 772-777. doi.org/10.26538/tjnpr/v5i4.29  http://www.doi.org/10.26538/tjnpr/v5i4.29
Copyright:
© 2021 Gbemisola et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

The roles of natural product in drug discovery and development cannot be over emphasised. It plays a vital role in human therapy and gives a better understanding on the cellular pathways.This study investigated the modulatory effects of partially purified fractions of Piper guineense Schumach. & Thonn. (Piperaceae), Zanthoxylum zanthoxyloides Lam. (Rutaceae), Amaranthus viridis L. (Amaranthaceae), Costus afer Ker-Gawl. (Zingiberaceae) and Catharanthus roseus (L.) G. Don. (Apocynaceae) on oestrogen receptor-? (ESR-?), tumour protein p53 (TP-53), retinoblastoma (RB) and NAD(P) H quinine oxidoreductase (NQO1) genes in cervical cancer cell line (HeLa cells). n-Hexane, ethylacetate, chloroform, and water fractions of 80% ethanol extracts of study plants were screened with brine shrimp lethality and water-soluble tetrazolium-1 (WST-1) cytotoxicity assay. HeLa cells were treated with 1:10 dilutions of IC50 concentrations of test fractions for 24 hours, total RNA was extracted, RNA quality was checked, and normalized to a baseline concentration. Gene expression was monitored by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results showed that ESR-? was downregulated (p < 0.05) by P. guineense-hexane, C. roseus-chloroform and A. viridis-ethylacetate fractions. TP53 gene was up-regulated (p < 0.05) by P. guineense-hexane and Z. zanthoxyloides-ethylacetate fractions. None of the test fractions caused an up-regulation in the expression of retinoblastoma gene. NQO1 gene was down-regulated (p < 0.05) by C. roseus-chloroform, P. guineense-hexane, A. viridis, C. afer, and Z. zanthoxyloides ethylacetate fractions. Our study provides scientific evidence of the possible anti-proliferative potentials of C. roseus-chloroform, P. guineense-hexane, ethylacetate fractions of A. viridis, C.afer, and Z. zanthoxyloides in cervical cancer.

Keywords: Natural products; Gene expression; rt-pcr; Tp53; Retinoblastoma; Oestrogen receptor-?; NAD(P)H Quinine Oxidoreductase
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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