Blumea balsamifera (L.) DC. Elicit Anti-Kinase, Anti-Phosphatase and Cytotoxic Activities against Acute Promyelocytic Leukemia Cells (HL-60)

Nurul A. Ismail1, Azlinah Matawali1, Ping-Chin Lee1, Siew-Eng How1, Boon H. Lee2, Lucky P. W. Goh1, Jualang A. Gansau1*
1Faculty of Science and Natural Resources, Universiti Malaysia Sabah, 88450 Kota Kinabalu, Sabah. Malaysia
2Drug Discovery Laboratory, Cancer Research Initiatives Foundation (CARIF), Subang Jaya, Selangor. Malaysia

Corresponding Author:; Tel: +60-88320000 ext. 100496
Recieved Date: 07 January 2021; Accepted Date: 07 April 2021; Published Date: 03 May
Citation: Ismail NA, Matawali A, Lee P-C, How S-E, Lee HB, Goh LPW, Gansau JA. Blumea balsamifera (L.) DC. Elicit Anti-Kinase, Anti-Phosphatase and Cytotoxic Activities against Acute Promyelocytic Leukemia Cells (HL-60). Trop J Nat Prod Res. 2021; 5(4): 656-660.
2021 Ismail et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Blumea balsamfera (L.) DC. (B. Balsamfera) extract has been shown to exhibit many biological activities. However, the anti-kinase, anti-phosphatase and cytotoxic activity of B. balsamfera are not well understood. Therefore, this study aimed to investigate the anti-kinase and anti-phosphatase activities using MKK1, MSG5 and PP1 screening systems. Cytotoxic activity was evaluated using acute promyelocytic leukaemia cell lines (HL-60). Methanol extracts of B. balsamfera were partitioned into hexane (HE), chloroform (CE), chloroform-methanol (CME), butanol (BE) and aqueous fractions (AQE). Only the CE fraction demonstrated toxic activity against PP1 screening system. Other fractions did not show activity in PP1 screening. CE fractions were further fractionated using silica gel chromatography and a further 11 fractions were obtained. Fraction 2 (CE.F2) showed activity against PP1 and was further fractionated and tested. CE.F2.F6.F3 fraction tested positive against PP1. Inhibition of PP1 by the F2.F6.F3 fraction was further confirmed using an enzymatic reaction and the Vmax and Km constants were 124.999 µmol/ml.min and 204.624 µM, respectively. A Lineweaver-Burk plot outcome of F2.F6.F3 revealed decreasing of Km and Vmax values which supported the inhibition of PP1 activities. Cytotoxic activities against HL-60 were observed for the CE, CE.F1, CE.F2 and CE.F7 fractions. We have demonstrated that B. balsamfera and its specific fractions exhibited anti-kinase and anti-phosphatase activities. These substances have the potential to be used as treatment agent for acute promyelocytic leukemia.

Keywords: Blumea balsamifera, anti-kinase, antiphosphatase, acute promyelocytic leukaemia
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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