Impact of Acidifier on Florfenicol Pharmacokinetics and their Tissue Residues in Escherichia coli O78-Infected Chickens

Maha S. Abd-Elhafeez1,4, Sherifa M. M. Sabra2,4, Nahla S. Elshater3,4*
1Chemistry Department, Toxicology and Feed Deficiency, Giza, Egypt
2Serology Unit and Bacterial Strains Bank, Giza, Egypt
3Reference Laboratory for Veterinary Quality Control on Poultry Production, Giza, Egypt
4Agricultural Research Center, Animal Health Research Institute, Giza, Egypt

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Recieved Date: 08 November 2020; Accepted Date: 05 April 2021; Published Date: 03 May
Citation: Abd-Elhafeez MS, Sabra SMM, Elshater NS. Impact of Acidifier on Florfenicol Pharmacokinetics and their Tissue Residues in Escherichia coli O78-Infected Chickens. Trop J Nat Prod Res. 2021; 5(4): 635-642.
© 2021 Abd-Elhafeez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Florfenicol (FF) is a broad-spectrum antibiotic and has been associated with high therapeutic efficacy and low toxicity; therefore, it is widely used in poultry farms. Acidifier has been reported to limit multiplication of enteropathogenic Escherichia coli. The study was conducted to investigate the effect of acidifier on pharmacokinetics of FF and also examine their tissue residues in E. coli O78-infected broiler-chickens. A total of 136 healthy broiler-chickens were used for the study. The broiler-chickens were grouped for pharmacokinetic (A-F), FF tissue residual (G-L) and colony forming unit (CFU; M-N) studies. They were infected with E. coli O78 and FF was administered orally for 3 consecutive days at 30 and 60 mg/kg, with / without acidifier. Faecal CFU of E. coli O78 was determined. At intervals of 1st, 3rd, 5th, 7th and 9th day post FF treatment, chickens were slaughtered and tissue specimens collected for analysis. High performance liquid chromatography (HPLC) was used to measure plasma of FF levels. The results showed that FF serum level was significantly lower in infected broiler-chickens compared with the healthy control group at the different time intervals. The outcome of the CFU showed a significant decrease in infected broiler-chickens with acidifier only (2.77±0.015 CFU/g) in the 4th day after infection in relation to those treated with FF, FF supplemented with acidifier (2.62±0.033 and 2.58±0.036 CFU/g, respectively) while in non-treated infected group was (4.00±0.008 CFU/g). Our findings recommend feed supplementation of acidifier (30 mg/kg BW) with FF for the treatment of E. coli O78-infected broiler-chickens.

Keywords: Acidifier, E. coli O78, Florfenicol, HPLC, Pharmacokinetics, Tissue residue.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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