Genotyping Toxins of Clostridium perfringens Strains of Rabbit and Other Animal Origins

Saba T. Hashim1*, Saad S. Fakhry2, Lubna M. Rasoul3, Tahreer H. Saleh1, Bahaa A. L. ALRubaii3
1Al-Mustansiriyah University, College of Science, Department of Biology, Baghdad, Iraq
2Ministry of Higher Education and Scientific Research, Science and Technology-Environment and water Directorate food contamination research center, Baghdad, Iraq
3University of Baghdad, Baghdad, College of Science, Department of Biology, Baghdad, Iraq

Corresponding Author:; Tel: 00964 771 204 6033
Recieved Date: 04 July 2020; Accepted Date: 07 April 2021; Published Date: 03 May

Hashim ST, Fakhry SS, Rasoul LM, Saleh TH, ALRubaii BAL. Genotyping Toxins of Clostridium perfringens Strains of Rabbit and Other Animal Origins. Trop J Nat Prod Res. 2021; 5(4): 613-616.

© 2021 Hashim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Clostridium perfringens is a pathogen of great economic importance, commonly known for toxin production that causes several diseases in animals and humans. Strain identification is necessary for accurate diagnosis of associated diseases caused by C. perfringens and the development of management strategies; hence toxin genotyping is more dependable than the traditional toxinotyping. Therefore, the present study was aimed at genotyping toxins of C. perfringens strains isolated from rabbit and other animals. A total of 60 strains of C. perfringens (19 from rabbit and 41 from cattle, sheep, goat and others animals) were isolated from infected spleens, livers and kidneys or intestinal contents. The isolates were identified using cultural and biochemical tests. Strain confirmation was done with a molecular biological approach. Multiplex and duplex PCR strategies were employed for genotyping C. perfringens strains by targeting the cpa and cpb2 genes, respectively. The results of toxin-genotyping and pathological profile distribution indicated that out of the 19 strains isolated from rabbit, the Type A genotype of C. perfringens, expressing the cpa gene was 63.16 %, while the cpa+cpb2 genotype was 36.84%. A similar finding was made for avian, ovine, and caprine isolates, with 90.90 % positive for the cpa gene and 9.10 % of the strains having the cpa+cpb2 genotype. Based on the findings, an effective management technique, viz a viz genotype style distribution of the C. perfringens cpa gene in the ecosystem, and strain genotyping is recommended.

Keywords: Clostridium perfringens, Genotype, PCR, Strain, Toxin.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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