In Vitro Antiamoebic Activity of Cnestis ferruginea and Dianthus basuticus on Entamoeba histolytica Trophozoites

Teslim O. Ojuromi1, Tom A. O. Ashafa1, Jesus N. Garza-Gonzalez2, Francisco Gonzalez-Salazar2,3*


1University of the Free State Qwaqwa Campus, Faculty of Natural and Agricultural Sciences, Department of Plant Sciences, Phuthaditjhaba, 9866, South Africa
2Departamento de Ciencias Básicas, Universidad de Monterrey, Av Ignacio Morones Prieto 4500, San Pedro Garza García, 66238, México
3Centro de Investigaciones Biomédicas Del Noreste, Instituto Mexicano Del Seguro Social, Monterrey, 64720, México

Corresponding Author: fgonz75@hotmail.com; Tel: 52- 81- 82533190
Recieved Date: 27 January 2021; Accepted Date: 12 March 2021; Published Date: 03 March
Citation: Ojuromi OT, Ashafa AOT, Garza-Gonzalez JN, Gonzalez-Salazar F. In Vitro Antiamoebic Activity of Cnestis ferruginea and Dianthus basuticus on Entamoeba histolytica Trophozoites. Trop J Nat Prod Res. 2021; 5(3):555-558. doi.org/10.26538/tjnpr/v5i3.22 http://www.doi.org/10.26538/tjnpr/v5i3.22
Copyright:
© 2021 Teslim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Amoebiasis is still a great disease burden in developing countries; this may cause around 100,000 deaths every year worldwide. The appearance of the strains of parasites resistant to conventional drugs used to treat this disease compels the search for new antiparasitic agents. As a result, a growing emphasis has been placed on plants used in traditional medicine to discover new agents with antiparasitic activity. The study determined the antiamoebic activity of Cnestis ferruginea and Dianthus basuticus found in Nigeria and South Africa. The assays were performed on cultures of Entamoeba histolytica growing in the logarithmic phase that were exposed to various concentrations of the ethanol extract of D. basuticus and C. ferruginea plants. The probit of the cultures were calculated, and the IC50 was determined for each extract for antiamoebic activity. The ethanol extract of both C. ferruginea and D. basuticus demonstrated a remarkable in-vitro inhibitory activity against E. histolytica trophozoites. The 50% inhibitory concentrations (IC50) of C. ferruginea and D. basuticus on E. histolytica cultures were 15.07 µg/mL and 12.45 µg/mL, respectively. The results showed that D. basuticus and C. ferruginea have inhibitory activity against E. histolytica.

Keywords: Dianthus basuticus, Cnestis ferruginea, Connaraceae, Dianthus, Entamoeba histolytica, Amoebiasis.
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