Effect of Epigallocatechin Gallate on Cadmium Chloride-Induced Changes in Behavior, Biochemical Parameters and Spermatogenesis of Male Sprague Dawley Rats

Subramani Parasuraman*, Lam C. Hui, James Y. K Beng, Brenda N. Y Qin


Faculty of Pharmacy, AIMST University, 08100 Bedong, Malaysia
Corresponding Author: parasuphd@gmail.com; Tel: +60 108826480
Recieved Date: 22 January 2021; Accepted Date: 15 March 2021; Published Date: 03 March
Citation: Parasuraman S, Hui LC, Beng JYK, Qin BNY. Effect of Epigallocatechin Gallate on Cadmium Chloride-Induced Changes in Behavior, Biochemical Parameters and Spermatogenesis of Male Sprague Dawley Rats. Trop J Nat Prod Res. 2021; 5(3):549-554. doi.org/10.26538/tjnpr/v5i3.21 http://www.doi.org/10.26538/tjnpr/v5i3.21
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Copyright: © 2021 Parasuraman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Epigallocatechin gallate (EGCG) is the ester of epigallocatechin and gallic acid and is a type of catechin.  EGCG is abundant in dry tea leaves and its ameliorative effect on heavy metal-induced behaviour and spermatogenesis is not clear. Hence, the present study is planned to study the ameliorative effect of EGCG on cadmium chloride (CdCl2)-induced changes in behaviour and spermatogenesis of Sprague Dawley rats. Healthy, male rats were divided into four groups viz., control, CdCl2 (5 mg/kg), CdCl2 (5 mg/kg) + vitamin C (200 mg/kg) and CdCl2 (5 mg/kg) + EGCG (50 mg/kg) respectively. All the animals were administered with the respective assigned treatment by intraperitoneal route for 28 days. During the study, body weights changes and the behavioral functions (locomotor activity, grip strength, and escape latency tendency) of the rats were monitored at weekly intervals.  At the end of the study, sperm samples were collected from cauda epididymis and motility was checked. Later, the number of spermatozoa was calculated using the red blood cell counting method. The animals administered with CdCl2 and CdCl2 + EGCG showed a significant reduction in body weight, behavioral functions and the number of sperm cells. Vitamin C prevented CdCl2-induced changes in behavioral functions and reduction in the number of sperm cells. In the present study, EGCG failed to prevent CdCl2-induced changes in the behavior of the rats and reduction in the number of sperm cells whereas, vitamin C has ameliorative effects on CdCl2-induced changes in behavioral functions and reduction in the number of sperm cells. 

Keywords: Behavioral functions, Biochemical parameter, Cadmium, Spermatozoa.
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