Assessment of Acute and Sub-Chronic Toxicity of HYK Aqueous Extracts in Experimental Animals

Quang Y. Pham1, Tien H. Vuong2, Minh T. Doan3, Hoang N. Nguyen4*

1Gia Lai Traditional Medicine and Rehabilitation Hospital, 13 Dang Thuy Tram street, Hoa Lu ward, Pleiku city, Gia Lai province 61109, Vietnam                    
2Hanoi Medical University, 1 Ton That Tung street, Dong Da district, Hanoi 11520, Vietnam
3Vietnam University of Traditional Medicine, 2 Tran Phu, Mo Lao, Ha Dong distritct, Hanoi 12110, Vietnam 
4Vietnam Military Medical University, 160 Phung Hung, Phuc La, Ha Dong distritct, Hanoi 12108, Vietnam



Corresponding Author: nganvmu@gmail.com; Tel: 0382689686
Recieved Date: 31 December 2020; Accepted Date: 3 March 2021; Published Date: 03 March
Citation: Pham QY, Vuong TH, Doan MT, Nguyen HN. Assessment of Acute and Sub-Chronic Toxicity of HYK Aqueous Extracts in Experimental Animals. Trop J Nat Prod Res. 2021; 5(3):519-527. doi.org/10.26538/tjnpr/v5i3.17 http://www.doi.org/10.26538/tjnpr/v5i3.17
Copyright:
© 2021 Quang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Hoa Yên Kinh (HYK) is an oriental prescription in Vietnamese Traditional Medicine that is used to treat premenopausal syndrome in the clinical stage. This study aimed to evaluate the safety, acute and sub-chronic toxicity of aqueous extracts of HYK in in vivo model. In the acute toxicity study, HYK at doses ranging from 150 to 300 g /kg/day was given to Swiss mice by oral administration. Mice were observed for general behavioural changes, adverse effects, and mortality up to 7 days post-treatment to find the highest dose that did not kill mice. In sub-chronic toxicity studies, HYK was given orally with doses of 12 g and 60 g/kg/day for 12 weeks. At the end of 12 weeks, the mice were operated to observe the whole organ for histopathology. The microscopic structures of the liver, spleen, and kidney of at least 30% of mice in each group was randomly checked. The acute toxicity study in all the doses used did not cause any significant change such that no LD50 was found. In addition, the sub-chronic toxicity study did not show any treatment-related abnormalities in the hematological and biochemical parameters. These results demonstrated that HYK aqueous extract is safe and does not appear to exert toxic effect.

Keywords: Traditional Vietnamese Medicine, HYK recipe, Premenopausal syndrome, Toxicity. 
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