Comparative Analysis of Phytochemicals and Antioxidant Activity of Ethanol Extract of Centella asiatica Leaves and its Nanoparticle Form

Bayyinatul Muchtaromah1*, Soraya Habibie2, Burhan Ma’arif3, Realsyah Ramadhan1, Evika S. Savitri1, Zahrotul F. Maghfuroh1
1Department of Biology, Faculty of Science and Technology, Maulana Malik Ibrahim State Islamic University, Malang 65151, Indonesia
2Department of Biology, UPBJJ Universitas Terbuka, Malang 65151, Indonesia
3Department of Pharmacy, Faculty of Medical and Health Science, Maulana Malik Ibrahim State Islamic University, Malang 65151, Indonesia

Corresponding Author: bayyinatul@bio.uin-malang.ac.id; Tel: +6281231842316
Recieved Date: 4 November 2020; Accepted Date: 8 March 2021; Published Date: 03 March
Citation: Muchtaromah B, Habibie S, Ma’arif B, Ramadhan R, Savitri ES, Maghfuroh ZF. Comparative Analysis of Phytochemicals and Antioxidant Activity of Ethanol Extract of Centella asiatica Leaves and its Nanoparticle Form. Trop J Nat Prod Res. 2021; 5(3):465-469. doi.org/10.26538/tjnpr/v5i3.9 http://www.doi.org/10.26538/tjnpr/v5i3.9
Copyright:
 © 2021 Muchtaromah et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

The high level of flavonoid compounds in pegagan (Centella asiatica) that has been found wildly in Indonesia is considered profitable.  The aims of this study were to perform comparative analysis of phytochemical and antioxidant activity of pegagan extract (PE) and pegagan nanoparticle form (PN), as well as predict compounds having antioxidant activity through metabolite profiling. C. asiatica leaves were extracted using 70% ethanol. Nanoparticle form was made from PE using ionic gelation method. Antioxidant activity was done using DPPH method, with ascorbic acid as positive control. The metabolite profiling process was done using liquid chromatography – mass spectrometry (LC-MS). The result from phytochemical test showed that there were 50 compounds identified in PE and 43 compounds identified in PN. The antioxidant activity in both samples was indicated by IC50 with 56.57 µg/mL for PE 44.67 µg/mL for PN. The findings showed that PN dosage form had better antioxidant activity than PE dosage form, proving that nanoparticle preparations from natural product had potential to be used in pharmaceutical dosage forms. The research concluded that compounds with known antioxidant properties were determined to be responsible for antioxidant activity in C. asiatica leaves from both PE and PN. 

Keywords: Antioxidant, Centella asiatica, Herbal, Nanoparticles
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