Evaluation of Analgesic, CNS Depressant and Antidiarrhoeal Activities of Psidium guineense Leaf Extract

Mahmuda Akhter1, Sayema Arefin2, Rabindra N. Acharyya3, Halima Akter1, Sadia Jahan1, Joy C. Rajbangshi1*
1Department of Pharmacy, Comilla University, Kotbari, Cumilla-3506, Bangladesh
2Department of Pharmacy, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh
3Pharmacy Discipline, Khulna University, Khulna-9208, Bangladesh

Corresponding Author: joyrajphr@gmail.com; Tel: +8801516754559
Recieved Date: 10 September 2020; Accepted Date: 9 March 2021; Published Date: 03 March
Citation: Akhter M, Arefin S, Acharyya RN, Akter H, Jahan S, Rajbangshi JC. Evaluation of Analgesic, CNS Depressant and Antidiarrheal Activity of Psidium guineense Leaves Extract. Trop J Nat Prod Res. 2021; 5(3):460-464. doi.org/10.26538/tjnpr/v5i3.8 http://www.doi.org/10.26538/tjnpr/v5i3.8
 © 2021 Akhter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Psidium guineense plant had been used as a folk medicine in Bangladesh for ages. The study evaluated the analgesic, central nervous system (CNS) depressant and antidiarrheal effects of aqueous (AEPG), methanol (MEPG) and dichloromethane (DEPG) extracts of the P. guineense leaves in mice. The analgesic activity was investigated by acetic acid-induced writhing test and tail immersion test whereas the CNS depressant and the antidiarrheal activity was assessed by hole cross test and magnesium sulphate-induced diarrhea in mice, respectively. The mice were given three doses of each extract (50, 100 and 200 mg/kg body weight) orally and 1% Tween-80 in normal saline (5 mL/kg) was used as control in all experiment. Ibuprofen (10 mg/kg), diazepam (2 mg/kg) and loperamide (10 mg/kg) were used as standard drugs in analgesic, CNS depressant and antidiarrheal tests, respectively. The extracts showed significant (p < 0.05) analgesic activity in both tests but DEPG (200 mg/kg) exhibited the maximum analgesic effect, 83.14% inhibition of writhing and 59.34% elongation of tail withdrawal time after 120 minutes. In the hole cross test, AEPG exerted the most depressant effect i.e.it reduced 97.64% of the movement of the mice after 120 min. MEPG at a dose of 200mg/kg inhibited diarrhoea by 95% and inhibited defeacation by 79.66%. Based on the results, it can be concluded that the leaf extracts of P. guineense possess marked analgesic, CNS depressant and antidiarrheal activities in mice.

Keywords: Psidium guineense, Analgesic, CNS depressant, Anti-diarrheal.
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