Distinctive Roles of Butylated Hydroxytoluene and Ascorbic Acid in Lead-Instigated Oxidative Stress in Wistar Rats

Esther N. O. Obiwulu1, Augustine Apiamu2*, Gilbert Ugbebor1
1Department of Integrated Science, Delta State College of Education, Agbor, Nigeria
2Department of Biochemistry, Faculty of Science, Delta State University, P.M.B. 1, Abraka, Nigeria

Corresponding Author: austodacademia.edu@gmail.com; Tel: +234(0)7060440357
Recieved Date: October 01, 2020; Accepted Date: January 24, 2021; Published Date: 03 February 2021
Citation: Obiwulu ENO, Apiamu A, Ugbebor G. Distinctive Roles of Butylated Hydroxytoluene and Ascorbic Acid in Lead-Instigated Oxidative Stress in Wistar Rats. Trop J Nat Prod Res. 2021; 5(1):135-139.  https://doi.org/10.26538/tjnpr/v5i1.19
Copyright: © 2021Obiwulu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Overwhelmed antioxidant defense system of exposed biological system to a toxicant maybe relieved using in vitro antioxidant source. Here, the study evaluated the distinctive roles of ascorbic acid (AA) and butylated hydroxytoluene (BHT) in lead (Pb)-instigated oxidative stress in Wistar rats, where group I rats received 1.0 mL of distilled water, group II rats received a single dose of 30 mg PbCl2 per kg b.w. of rats by intraperitoneal injection, groups III and IV dosed with the toxicant received 25 mg/kg of AA and BHT, and group V dosed with the toxicant received 25 mg/kg of AA and BHT orally daily for 28 days. There was a significant increase (p < 0.05) in serum, hepatic and renal MDA levels for group II rats relative to the group I rats, but this was reversed in tissues of groups III-V rats relative to group II rats. No significant changes (p > 0.05) occurred in tissue MDA levels for group III and IV rats. SOD activity decreased significantly for group II rats, as compared with group I rats, but group III-V rats showed enhanced SOD activity in tissues assessed in relation to group II rats at p<0.05. CAT activity was significantly inhibited in tissues of group II rats relative to group I rats, which was significantly improved in group III-V rats relative to group II rats at p<0.05. Therefore, Pb-mediated oxidative stress was better improved by oral administration of AA than BHT, which suggested the exogenous use of natural antioxidants in lead toxicity.

Keywords: Antioxidant, Ascorbic acid, Butylated hydroxytoluene, Lead toxicity, Oxidative stress.
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