Sterculia foetida Leaf Fraction Against Matrix Metalloproteinase-9 Protein and 4T1 Breast Cancer Cells: In-Vitro and In-Silico Studies

Rollando Rollando1,2*, Warsito Warsito3, Masruri Masruri3, Widodo Widodo4

1Pharmacy Department, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia
2Doctoral Student, Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
3Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia
4Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, Indonesia

Corresponding Author: ro.llando@machung.ac.id; Tel: +6282220379864
Recieved Date: December 06, 2020; Accepted Date: February 03, 2021; Published Date: 03 February 2021
Citation: Rollando R, Warsito W, Masruri M, Widodo W. Sterculia foetida Leaf Fraction Against Matrix Metalloproteinase-9 Protein and 4T1 Breast Cancer Cells: In-Vitro and In-Silico Studies. Trop J Nat Prod Res. 2021; 5(1):113-121.  https://doi.org/10.26538/tjnpr/v5i1.15
Copyright: © 2021 Rollando et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Sterculia foetida leaf extract has been shown to have cytotoxic activity. Matrix metalloproteinase-9 (MMP-9) has an important role in pathophysiological functions. Inhibition of MMP9 is an important therapeutic approach for combating cancer. This study was conducted to determine the most active fraction of S. foetida as anti-breast cancer agent with hemopexin-like domain of MMP-9 (PEX9) as the selective protein target and 4T1 cells line as metastatic breast cancer cell. The leaves S. foetida was extracted using 80% methanol and was fractionated into fractions of n-hexane, chloroform, ethyl acetate, n-butanol, and insoluble n-butanol with liquid-liquid partition. In vitro screening against MMP-9 was performed using FRET-based assay and cytotoxic tests were performed using the MTT assay. Identification of compounds in the most active fraction using GC-MS. The docking to PEX9 was run using AutoDock Vina embedded in PyRx program. The n-hexane fraction was the most active fraction to inhibit MMP-9 with an IC50 of 19.67 µg/mL and inhibit the growth of 4T1 cells with an IC50 of 34.65 µg/mL. NNGH was used as positive control for the in-vitro and in-silico studies. The GC-MS results of the n-hexane fraction showed that there were 23 compounds, and they had binding affinity score of -8.9 to -4.9 kcal/mol towards PEX9. It can be concluded that S. foetida leaf has the potential to be developed for therapeutic use, especially for breast cancer therapy.

Keywords: Sterculia foetida, Fraction, Cytotoxic, MMP-9, 4T1, PEX9.
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