In vivo Anti-Inflammatory Activity of Derris reticulata Ethanol Extract

Krittiya Thisayakorn1, Nantiya Joycharat2,3, Thaweeporn Keereekoch2, Bodin Chatawatee2, Katesarin Maneenoon2, Sasitorn Chusri2,3, Nongluk Kunworarath2*

1Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technology Research (TISTR), Techno Polis, Thailand
2Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand
3Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand

Corresponding Author: nonglukkunworarath@gmail.com; Tel: +66-74-282722
Recieved Date: November 23, 2020; Accepted Date: January 26, 2021; Published Date: 03 February 2021
Citation: Thisayakorn K, Joycharat N, Keereekoch T, Chatawatee B, Maneenoon K, Chusri S, Kunworarath N. In vivo Anti-Inflammatory Activity of Derris reticulata Ethanol Extract. Trop J Nat Prod Res. 2021; 5(1):100-104.  https://doi.org/10.26538/tjnpr/v5i1.13
Copyright: © 2021 Thisayakorn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Derris reticulata Craib. has been used in Thai traditional medicine to treat various inflammatory diseases. Recently, D. reticulata extract has been reported to exert anti-inflammatory activity by inhibiting nitric oxide (NO) secretion and the expression of various mRNAs in stimulated macrophage cells. However, studies of its anti-inflammatory effects in an animal model have not been performed. The study evaluated the in vivo anti-inflammatory activity of an ethanol extract of the wood of D. reticulata (DRE). The anti-inflammatory effect of DRE on ethyl phenylpropiolate (EPP)-induced ear edema and carrageenan-induced paw edema in rats was studied. Furthermore, the NO scavenging activity and phytochemical contents of the DRE were determined. The results showed that the most abundant phytochemical compound was lupinifolin, followed by total proanthocyanidin, total phenolic compounds, and total flavonoids. DRE also exhibited NO scavenging activity (15.8 - 49.7%) while 1%, 5%, and 10% DRE promoted a significant reduction of inflammation in EPP-induced rat ear edema (35.0 - 52.5%) and carrageenan-induced rat paw edema (28.1 - 41.0%). The study revealed that DRE possesses potent anti-inflammatory properties in animal models. The results confirm the efficacy of the traditional use of D. reticulata in the treatment of inflammatory diseases.

Keywords: Derris reticulata, Anti-inflammatory activity, Ear edema, Paw edema, In vivo studies
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