Evaluation of the Antiglycation Effect of Branched Chain Amino Acids and Phytochemical Compounds on RAW 264.7 Cell Line and their Synergistic Effect on Colorectal Cancer Cell Line Panel

Moath Alqaraleh1*, Violet Kasabri2, Ahmad Aljaafreh3, Ibrahim Al-Majali3, Nihad Al-Othman4, Khaled Khleifat5, Sara Khaleel6, Nafe M. Al‐Tawarah3, Haitham Qaralleh3, Mohammad Al-majali7, Israa Yousef1, Arwa Al-Khatib1, Alia S. Khwaldeh8

1Pharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan
2Department of Pharmacy, Faculty of Pharmacy. The University of Jordan, Amman, Jordan
3Department of Medical Analysis, Mutah University, Mutah, Karak, 61710, Jordan
4Division of Anatomy, Biochemistry and, Genetics. Faculty of Medicine and Health Sciences, An-Najah National University, Nablus
5Department of Biology, Mutah University, Karak, Mutah, 61710 Jordan
6Faculty of Pharmacy, Zarqa University, P.O. Box 132222, Zarqa, 13132, Jordan
7Department of Biological Sciences, Faculty of Science. The University of Jordan, Amman, Jordan
8Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid, Jordan

Corresponding Author: m.alqaraleh@ammanu.edu.jo; Tel: ‎+962 0795038456
Recieved Date: November 20, 2020; Accepted Date: January 27, 2021; Published Date: 03 February 2021
Citation: Alqaraleh M, Kasabri V,  Aljaafreh A, Al-Majali I, Al-Othman N, Khleifat  K, Khaleel S, Al‐Tawarah NM, Qaralleh H, Al-majali M, Yousef  I, Al-Khatib A, Khwaldeh AS. Evaluation of the Antiglycation Effect of Branched Chain Amino Acids and Phytochemical Compounds on RAW 264.7 Cell Line and their Synergistic Effect on Colorectal Cancer Cell Line Panel. Trop J Nat Prod Res. 2021; 5(1):88-93. https://doi.org/10.26538/tjnpr/v5i1.11
Copyright: © 2021 Alqaraleh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Flavonoids and branched chain amino acids (BCAAs) have been recognized as attractive agents for research due to their role in diabetes and cancer. The present study investigates the antiglycation effect of BCAAs and flavonoid compounds and their cytotoxic effect on different colorectal cancer cell lines individually and in combination. This study was conducted by using RAW 264.7 cell line (murine monocyte-macrophage cell and Methylglyoxal (MGO) for inducing the glycation effect. The Sulforhodamine B (SRB) assay was used to study the cytotoxicity of BCAAs and flavonoids compounds using colorectal cell lines SW480, SW620 and CACO2.Flavonoids showed significant antiglycation effect compared to Aminoguanidine (AMG), while none of the BCAAs compounds displayed antiglycation effect. All flavonoids and branched-chain amino acids showed less degree of cytotoxicity against SW620, CACO2 and SW480 as compared to cisplatin. The synergetic effects was only shown against SW620 and CACO2 cell lines by using the combination of Apigenin and BCAAs while antagonistic effect appeared when combination of Quercetin and Hesperidin were used against SW480, SW620 and CACO2 cell lines. Flavonoids such as Quercetin, Apigenin and Hesperidin could be used as a remedy for diabetes due to their surpassing effect upon antiglycation action of AMG.

Keywords: Flavonoids, BCAAs, Antiglycation, Cytotoxicity, Co-incubation. 
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