Ulcer-Protective Property of Bryophyllum pinnatum Leaf Extract and their Phytosomal Formulations 

Emenike B. Amadi1, Emmanuel C. Ossai1*, Obiora C. Ugwu2, Valentine O. Nwanelo1, Chioma A. Anosike1, Petra O. Nnamani3, Okwesili F. C. Nwodo1
1Ethnopharmacology, Food and Drug Delivery Research Unit, Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria
2Department of Pharmacology, Faculty of Pharmaceutical Sciences, Enugu State University of Science and Technology, Enugu State, Nigeria
3Drug Delivery and Nanomedicine Research Group, Public Health and Environmental Sustainability Research Group, Department of Pharmaceutics Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria

Corresponding Author: emmanuel.ossai@unn.edu.ng; Tel: +2347038514389
Recieved Date: October 13, 2020; Accepted Date: December 23, 2020; Published Date: 02 January 2021
Citation: Amadi EB, Ossai EC, Ugwu OC, Nwanelo VO, Anosike CA, Nnamani PO, Nwodo OFC. Ulcer-Protective Property of Bryophyllum pinnatum Leaf Extract and their Phytosomal Formulations. Trop J Nat Prod Res. 2020; 4(12): 1201-1207. https://doi.org/10.26538/tjnpr/v4i12.29
Copyright: © 2020 Amadi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Active phytoconstituents usually exhibit robust in vitro pharmacological properties that do not correspond proportionately to the in vivo clinical benefits owing to their low gastrointestinal absorption.  To improve on the bioavailability of the plant natural products, a strategy to enhance their in vivo absorption is developed via novel formulation of phytosome – a phospholipid-phytoconstituent complex. This study formulated Bryophyllum pinnatum extract into phytosome, evaluated and compared the ulcer-protective properties of the phytosome and the Bryophyllum pinnatum free extract in indomethacin-induced ulcerative animal ulcer model. Phytosomal formulations of Bryophyllum pinnatum leaves were prepared by combination of ethanol extract of Bryophyllum pinnatum and phosphatidylcholine (phospholipon® 90H) in different ratios of 1:1, 1:3, and 1:5, respectively. The phyto-phospholipid complexes were evaluated for some physical and chemical properties, which include entrapment efficiency, Fourier transform infrared (FTIR) spectroscopy and in vitro drug release. Standard laboratory methods were employed in the phytosome formulation, evaluation and ulcer-protective studies. Ulcer protective studies were carried out using 400 mg/kg of phytosome and crude extract. All the phytosomal formulations showed increased drug release profile in phosphate buffer (pH 6.8) when compared to the free extract. At 400 mg/kg, the complexes significantly (p < 0.05) improved ulcer-protective property and oxidative stress parameters when compared to the free extract, and hence, could be a better form for ulcer management.

Keywords: Bryophyllum pinnatum, Phytosome, In vitro drug release, Ulcer inhibition.
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