Investigation of the Antidiabetic Activity and GC-MS Analysis of Extracts of Lilium polyphyllum

Amin M. Mir1, Muhammad W. Ashraf2, Swarn P. Singh3, Shailendra P. Madwal4, Bilal A. Mir5
1Assistant Research Professor, Department of Mathematics & Natural Sciences, Prince Mohammad Bin Fahd University, AlKhobar, Saudi Arabia 
2Dean Department of Mathematics & Natural Sciences, Prince Mohammad Bin Fahd University, AlKhobar, Saudi Arabia
3Research Scholar, IIIM Jammu (Jammu and Kashmir) India
4Professor Dept. of Chemistry, Govt. (PG) College Jaiharikhal Pauri Garhwal (Uttarakhand) India
5Assistant Professor, Department of Environmental Sciences & Microbiology UCBMSH Dehradun Uttarakhand India

Corresponding Author: mohdaminmir@gmail.com; Tel: +91-9897635334
Recieved Date: July 05, 2020; Accepted Date: December 23, 2020; Published Date: 02 January 2021
Citation: Mir MA, Ashraf MW, Singh SP, Madhwal SP, Mir BA. Investigation of the Antidiabetic Activity and GC-MS Analysis of Extracts of Lilium polyphyllum. Trop J Nat Prod Res. 2020; 4(12):1190-1195.  https://doi.org/10.26538/tjnpr/v4i12.27
Copyright: © 2020 Mir et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Diabetes is a disease characterized by high blood glucose (hyperglycaemia) due to insufficient insulin production or impaired tissue response to insulin. The hormone Insulin, produced in the pancreas, helps in the regulation of blood glucose. The present study is aimed at finding an alternative approach to blood glucose regulation by the use of Lilium polyphyllum Stem extract as potential inhibitor of the enzymes; ?-amylase and ?-glycosidase, which are responsible for the breakdown of oligosaccharides into glucose molecules. The ?-amylase and ?-glycosidase inhibitory activities of the extracts of Lilium polyphyllum in vitro using standard procedures. The ethanol extract of the plant was subjected to gas chromatographic-mass spectrometric (GC-MS) analysis. The results showed inhibition of ?-amylase by 54.95% (water extract), 53.01% (ethanol extract) and 47.87% (dichloromethane extract). The inhibition of ?-glucosidase was found to be 48.48 %, 48.48 % and 43.85 % for water, ethanol and dichloromethane extracts, respectively. The possible compounds that could be responsible for the enzyme inhibition were identified by GC-MS analysis as; Methyl 2-furoate, 5-hydroxymethyl furfural, Piperine, Palmitic acid, and Methyl Palmitate. The results revealed that the extracts of Lilium polyphyllum could be a possible source of natural antidiabetic medicine.

Keywords: Antidiabetic, Lilium polyphyllum, Alpha amylase, Alpha glycosidase.
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