Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species

Toluwanimi V. Afolayan*, Moses E. Adah, Olubunmi I. Ibidapo, Moses O. Orotope, Frank A. Orji, Ariyo C. Famotemi, Joy Ehiwuogu-Onyibe, Adekunle K. Lawal
Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
Corresponding Author: afolayantoluwanimi@gmail.com; Tel: +2348067993673
Recieved Date: September 08, 2020; Accepted Date: December 16, 2020; Published Date: 02 January 2021
Citation: Afolayan TV, Adah ME, Ibidapo OI, Orotope MO, Orji FA, Famotemi AC, Ehiwuogu-Onyibe J, Lawal AK. Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species. Trop J Nat Prod Res. 2020; 4(12): 1178-1181.  https://doi.org/10.26538/tjnpr/v4i12.25
Copyright: © 2020 Afolayan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Tannase is produced extensively from microorganisms such as fungi, bacteria and yeast as they are diverse naturally. The aim of this study was to isolate, screen and select local microbial strain for the production of tannase as well as optimize culture conditions for the hyper producers. Fifteen fungal species were isolated from decomposed tannin rich materials (soil under Almond leaves undergoing natural deterioration) and its surrounding soil. They were screened with mineral medium compounded with tannic acid. Selected isolates were used for tannase production under solid state fermentation and varied the fermentation conditions. All strains showed various degree of tannic acid utilization as carbon source for enzyme (tannase) production. Three isolates were identified to be potential hyper producers. The fungal species; Aspergillus niger, Penicillum sp. and Aspergillus flavipes yielded the highest enzyme production after incubation. Optimum temperature and pH for tannase production was recorded as 28-45°C and 5.5 respectively. The isolates were good tannase producer with high activities of the enzyme, especially Aspergillus niger, Penicillum sp. and Aspergillus flavipes which gave a high yield of tannase. 

Keywords: Tannase, Fermentation, Culture conditions, Optimum temperature, Optimum pH, Tannin.
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