In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

Oluwakemi A. Bello1*, Olatunde Fajimi2, Edward B. Esan3, Olawole O. Obembe1
1Department of Biological Sciences, College of Science and Technology, Covenant University, P. M. B 1023 Canaanland Ota, Ogun State, Nigeria
2Biotechnology unit, National Centre for Genetic Resources and Biotechnology, Moor Plantation, Ibadan, Oyo State. Nigeria
3G.P.O 2742 Dugbe, Ibadan, Oyo State. Nigeria


Corresponding Author: adetutu.bello@covenantuniversity.edu.ng; Tel: +2348033308445
Recieved Date: September 08, 2020; Accepted Date: December 24, 2020; Published Date: 02 January 2021
Citation: Bello OA, Fajimi O, Esan EB, Obembe OO. In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis. Trop J Nat Prod Res. 2020; 4(12):1174-1177.  https://doi.org/10.26538/tjnpr/v4i12.24
Copyright: © 2020 Bello et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Solanecio biafrae (Olive & Hierne) C. Jeffrey (Asteraceae) is a tropical, underutilized African indigenous vegetable, highly nutritious and medicinal. It grows as undercover and is mostly collected in tree plantations such as cocoa and kola. Its propagation is seldom carried out by vines, but its climbing habit poses a problem for farmers, which leads to the application of herbicide to control their spread. In order to ascertain its potential for genetic improvement, the protocol for direct shoot organogenesis of S. biafrae was developed. Leaf lamina, petiole, and leaf with petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP) alone and in combination with Indole butyric acid (IBA). Direct shoot organogenesis was obtained from the cut end of leaf lamina and petiole explants as well from the petiolar region of the leaf with petiole explant. The results obtained from this study show the potential of these explants of S. biafrae to serve as culture material for its micropropagation, genetic improvement via transgenesis. Hence, this regeneration protocol of S. biafrae can be coupled to its genetic transformation to improve species with shorter internode and more leaves to enhance the structure of tropical vegetation.

Keywords: Solanecio biafrae, Woorowo, Bologi, African Indigenous Vegetable, Micropropagation.
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