UPLC-MS Profile and Anti-Proliferative Activity of the Berries of an Aggressive Wild-Growing Weed: Solanum elaeagnifolium Cav. (Solanaceae)

Khawla D. Al-Hamaideh1*, Isra Dmour2, Tamam El-Elimat3, Fatma U. Afifi4,5
1Department of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, Al-Salt 19117, Jordan
2Department of pharmacy, Faculty of Pharmacy, Zarqa University, Zarqa, Jordan  
3Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan
4Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Applied Science University, Amman, Jordan
5Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan

Corresponding Author: kawladamen@bau.edu.jo; Tel: +962795776553
Recieved Date: October 29, 2020; Accepted Date: December 20, 2020; Published Date: 02 January 2021
Citation: Al-Hamaideh KD, Dmour I, El-Elimat T, Afifi FU.UPLC-MS Profile and Anti-proliferative Activity of the Berries of an Aggressive Wild-growing Weed: Solanum elaeagnifolium Cav. (Solanaceae).Trop J Nat Prod Res. 2020; 4(12): 1131-1138.  https://doi.org/10.26538/tjnpr/v4i12.16
Copyright: © 2020 Al-Hamaideh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Although,   Solanum elaeagnifolium Cav. is described as a problematic weed difficult to eradicate, it is used in the traditional medicine of different countries for the treatment of different ailments, such as tooth ache and constipation. Its occurrence in Jordan is widespread.    In the present study, the aim was to identify the phytochemical composition of the berries and to screen for different biological activities. Phytochemically, the hydro-alcoholic extracts of the berries of S. elaeagnifolium were analyzed using Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). Pharmacologically, using Sulforhodamine B (SRB) assay, the anti-proliferative effect of the extracts against four colorectal cancer cell lines (HT29, HCT116, SW620 and CACO2) was tested. The aqueous extracts of the ripe and unripe berries were further evaluated for their in vitro anti-lipidemic and anti-obesity activities. UPLC-MS analysis resulted in the identification of seven bioactive compounds in both extracts. Three of them, namely; apigenin, hyperoside and luteolin were identified in these berries for the first time. Also, chlorogenic acid, kaempferol, solasodine and solamargine were identified. The extracts exhibited anti-proliferative activity but lacked anti-obesity activity in vitro. This is the first report on the phytochemical and pharmacological evaluation of the ripe and unripe berries of S.  elaeagnifolium Cav., growing wild in Jordan.

Keywords: Colorectal cancer, Jordan, Obesity, Solanum elaeagnifolium Cav., Solanaceae, UPLC-MS.  
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